1μg (R: reducing condition, N: non-reducing condition).
4-1BB Ligand/TNFSF9 His Tag Protein, Human
4-1BB Ligand/TNFSF9 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD137L |
| Accession | P41273 |
| Amino Acid Sequence | Arg71-Glu254 with His Tag at the N-Terminus |
| Expression System | E.coli |
| Molecular Weight | 18-22kDa(R) |
| Purity | ＞95% by SDS-PAGE & ＞90% by SEC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, Ph7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Cancer Res.2019 Feb 15;79(4):783-794. |
Background
The 4-1BB ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that is part of the TNF superfamily. 4-1BBL is the high affinity ligand of 4-1BB. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD).
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SDS-PAGE
SEC-HPLC
The purity of 4-1BB Ligand/TNFSF9 His Tag Protein, Human is greater than 90% as determined by SEC-HPLC.
ELISA
Immobilized 4-1BB/CD137 Fc Chimera Protein, Human (Cat. No. UA010653) at 2.0μg/mL (100μL/well) can bind 4-1BB/Ligand/TNFSF9 Protein, Human (Cat. No. UA011045) with EC50 of 2.61-3.44 ng/ mL.
