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25-OH VD3 Recombinant Rabbit mAb (SDT-321-345)

25-OH VD3 Recombinant Rabbit mAb (SDT-321-345)

Catalog Number: S0B3602 Application: ELISA Brand: Starter
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Regular price $1,085 USD
Regular price Sale price $1,085 USD
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Product Details

Product Specification


Host Rabbit
Antigen 25-OH VD3
Synonyms Calcifediol, 25-Hydroxy Vitamin D3, 25-Hydroxycholecalciferol
Clone Number SDT-321-345
Antibody Type Recombinant mAb
Isotype IgG
Application Competitive ELISA
Cross Reactivity

It also reacts with 25-OH-VD2

Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt, 2 to 8 °C as supplied

Background

25-hydroxyvitamin D3 (25-OH D3) is the major circulating form of vitamin D and the preferred biomarker for assessing overall vitamin D status. It is measured in serum or plasma, typically via immunoassay or liquid chromatography-tandem mass spectrometry (LC-MS/MS). This diagnostic test quantifies the body's vitamin D reserves, reflecting both cutaneous synthesis and dietary intake. It is crucial for evaluating deficiency, insufficiency, sufficiency, or toxicity. Results guide clinical decisions in managing bone health (e.g., osteoporosis, rickets), and are increasingly used in research linking vitamin D to immune, cardiovascular, and metabolic disorders. Consistent and accurate measurement remains essential for reliable diagnosis and therapeutic monitoring.

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Compete ELISA

Competitive ELISA binding of 25-OH-VD3 recombinant mAb (S0B3602) with DNP-BSA conjugate.
Free 25-OH VD3 blocks 25-OH-VD3 recombinant mAb (S0B3602) binding with coating antigen (VD3-BSA conjugate)
Coating antigen: VD3-BSA conjugate, 0.5 µg/ml
S0B3603: 400 ng/ml
Free 25-OH-VD3 dilutions start from 200 ng/ml.
IC50= 4.043 ng/ml.