WB result of P4HB Recombinant Rabbit mAb
Primary antibody: P4HB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 25 µg
Lane 2: HeLa whole cell lysate 25 µg
Lane 3: Hl-60 whole cell lysate 25 µg
Lane 4: THP-1 whole cell lysate 25 µg
Lane 5: SK-OV-3 whole cell lysate 25 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDa
P4HB Recombinant Rabbit mAb
P4HB Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | P4HB |
| Synonyms | Protein disulfide-isomerase; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; ERBA2L; PDI; PDIA1; PO4DB |
| Location | Cell membrane, Melanosome, Endoplasmic reticulum |
| Accession | P07237 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Hep G2, HeLa, HL-60, THP-1, SK-OV-3, NIH/3T3, mouse liver, mouse pancreas, rat liver |
| Purification | Protein A |
| Concentration | 1.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:400-1:800 | Hu, Ms, Rt |
Background
P4HB, also known as protein disulfide isomerase (PDI) or PDIA1, is a multifunctional endoplasmic reticulum (ER) protein with a molecular weight of approximately 57 kDa. It contains four thioredoxin-like domains (a, b, b′, and a′), with the a and a′ domains harboring the catalytic CGHC motif. Adopting a horseshoe-shaped conformation and bearing a KDEL ER-retention signal at its C-terminus, it is one of the most abundant marker proteins in the ER. As the β subunit of the prolyl 4-hydroxylase complex, it forms an α2β2 tetramer with α subunits and participates in the 4-hydroxylation of proline residues in procollagen, which is essential for stabilizing the collagen triple helix. Meanwhile, functioning as a disulfide isomerase/oxidoreductase, it catalyzes the formation, breakage, and rearrangement of disulfide bonds in nascent secretory and membrane proteins, thereby maintaining ER protein folding and quality control. P4HB also exhibits concentration-dependent chaperone activity, inhibiting the aggregation of misfolded proteins at high concentrations or after phosphorylation by FAM20C. Additionally, it can bind thyroid hormones, participate in nitric oxide transport via S-nitrosothiols, and serve as a structural subunit of the microsomal triglyceride transfer protein complex. Beyond its ER functions, a fraction of P4HB is present on the cell surface or extracellularly, where it regulates the redox state of surface proteins such as integrins, contributing to platelet activation, fibrin formation, and thrombosis, and is implicated in pathological processes including tumor progression, neurodegenerative diseases, and Cole-Carpenter syndrome.
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Picture
Western Blot
WB result of P4HB Recombinant Rabbit mAb
Primary antibody: P4HB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 25 µg
Lane 2: mouse liver lysate 25 µg
Lane 3: mouse pancreas lysate 25 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
WB result of P4HB Recombinant Rabbit mAb
Primary antibody: P4HB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 25 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 54 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-P4HB antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
