Neutrophil Elastase Recombinant Rabbit mAb (S-4397-22)

Catalog Number: S0B60719 Application: WB, IHC-P, IF Reactivity: Ms Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Neutrophil Elastase
Synonyms	Neutrophil elastase; Elastase-2; Leukocyte elastase; Ela2; Elane
Immunogen	Synthetic Peptide
Location	Nucleus, Lysosome
Accession	Q3UP87
Clone Number	S-4397-22
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IF
Reactivity	Ms
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms
IHC-P	1:1000	Ms
ICC	1:500	Ms

Background

Neutrophil elastase (NE; gene ELANE, also known as ELA2) is a 218-amino-acid, ~29–33 kDa chymotrypsin-like serine protease that is synthesized in promyelocytes and stored in the azurophilic (primary) granules of neutrophil granulocytes. It is released upon neutrophil activation and degrades elastin as well as collagen, fibronectin, proteoglycans, and other matrix and plasma proteins, giving it a central role in antimicrobial defense, inflammatory tissue injury, and matrix remodeling. Its proteolytic activity is tightly controlled by endogenous inhibitors, chiefly α1-antitrypsin (SERPINA1), and inherited ELANE mutations cause severe congenital neutropenia, while unchecked elastase activity contributes to emphysema, ARDS, and chronic inflammatory lung disease. In diagnostic pathology, NE is used as a sensitive immunohistochemical marker of neutrophil/myeloid lineage (e.g., clone NP57), helpful in identifying neutrophilic infiltrates, myeloid leukemia, and neutrophil-rich skin or soft-tissue lesions.

Picture

Western Blot

WB result of Neutrophil Elastase Recombinant Rabbit mAb
Primary antibody: Neutrophil Elastase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse bone marrow lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 30 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse bone marrow. Anti-Neutrophil Elastase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Neutrophil Elastase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse brain. Anti- Neutrophil Elastase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded mouse bone marrow. Anti- Neutrophil Elastase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

Negative control: IF shows negative staining in paraffin-embedded mouse brain. Anti- Neutrophil Elastase antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.