Measured in a cell proliferation assay using LOVO cells, The EC50 for this effect is less than 0.5μg/ml.
Leptin Protein, Human
Leptin Protein, Human
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Regular price
$30 USD
Regular price
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$30 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | LEPD, Obesity protein (OB), LEP |
| Accession | P41159 |
| Amino Acid Sequence | Val22-Cys167 |
| Expression System | E.coli |
| Molecular Weight | 16kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 80mM NaCl, 5%trehalose, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Handbook of Biologically Active Peptides (Second Edition), 2013, Pages 1251-1256 2.Growth Factors and Their Receptors in Cell Differentiation, Cancer and Cancer Therapy, 2011, Pages 87-104 |
Background
Leptin (LEP) is a 16kDa peptide hormone produced mainly by white adipocyte tissue. Leptin is involved in maintaining gastric epithelial cell integrity and gastroprotection. In rats, systemic leptin is effective in attenuating both ethanol- and aspirin-induced damage to the gastric mucosa. This is correlated with an increase of leptin production by the stomach during experimentally-induced gastric damage in rats and during H pylori infection in humans. This gastric cytoprotective effect of leptin involves an increase in blood flow, local production of nitric oxide and prostaglandin E2, and vagus nerve-dependent mechanisms. One of the important functions of leptin is its role in the regulation of inflammatory processes. There is indeed a large body of evidence that leptin-mediated signal pathways play an active role in innate and adaptive immunity through alteration of various target genes transcription.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
ELISA
Immobilized Leptin, Human (Cat. No. UA040148) at 10.0μg/mL (100μL/well) can bind Leptin R/CD295 His Tag, Human (Cat. No. UA010682) with EC50 of 0.96-1.29 μg/mL.
