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Human TNF-α ELISA Kit

Human TNF-α ELISA Kit

Catalog Number: abs510006 Application: ELISA Reactivity: Human Conjugation:
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$450 USD
$450 USD
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Product Details

Product Specification

Species Reactivity Human
Theory This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).
Synonym APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha
Composition Please use it within the validity period of the kit (new and old products are shipped randomly)
form Specifications Shelf life of diluted or redissolved reagent after unpacking
Human TNF-α Microplate 1 piece Unused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant
Human TNF-α Standard 2 sticks After dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days
Human TNF-α detection antibody 1 stick After dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days
40 × SA-HRP 1 stick 40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage
10 × Buffer for concentration and dilution 1 vial After opening, it can be stored at 2-8 °C for 30 days
Chromogenic liquid 1 vial
Stop liquid 1 vial
20 × concentrated wash buffer 1 vial
Sealing film 3 sheets Stored at room temperature, to avoid contamination, not reusable
Background Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa.
TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α.
General Notes 1. Please use the kit within the validity period.
2. The components of different kits and different batch kits cannot be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.
4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.
Storage Temp. Kit unopened, stored at 2-8 °C.
Test Range 15.6pg/mL-1000pg/mL

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