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Human IL-2 ELISA Kit(Advanced)

Human IL-2 ELISA Kit(Advanced)

Catalog Number: abs510035 Application: ELISA Reactivity: Human Conjugation:
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$397 USD
$397 USD
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Product Details

Product Specification

protein IL-2
WGK Germany 0
Beilstein 0
Species Reactivity Human
Theory This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IL-2 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, IL-2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).
PubChem CID 0
Wells 0
Composition Please use within the expiration date of the kit
form Specifications Shelf life of diluted or redissolved reagent after unpacking
Human IL-2 Microplate 1 piece Unused slats can be stored at 2-8 °C for 30 days after being sealed back in aluminum foil bags with desiccant
Human IL-2 Standard 2 sticks After dissolution, the calculated amount is dispensed and stored at-20 °C for 14 days
Human IL-2 Detection antibodies 1 stick After dissolution in concentrated volume, it can be stored at 2-8 °C for 14 days
40 × SA-HRP 1 stick 40 × concentration can be stored at 2-8 ° C.; 1 × working concentration Not recommended for storage
10 × Buffer for concentration and dilution 1 vial After opening, it can be stored at 2-8 °C for 30 days
Chromogenic liquid 1 vial
Stop liquid 1 vial
20 × concentrated wash buffer 1 vial
Sealing film 3 sheets Stored at room temperature, to avoid contamination, not reusable
Background Human interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is an α-helix polypeptide with a molecular weight of 15-18kDa with different glycosylations, which belongs to the common γ-chain (γc) cytokine family members. It exists as a monomer with a very short half-life (< 30min). The precursor of human IL-2 has 153 amino acids, including a 20 amino acid signal sequence, and a 133 amino acid mature polypeptide. IL-2 mature protein contains an O-type glycosylation site at threonine position 3 and three cysteines, of which the intra-chain disulfide bond formed by two cysteines is essential for IL-2 activity. The mature human IL-2 amino acid sequence shares 73%, 66%, 78%, and 97% homology to IL-2 in dog, rat, cat, and macaque, respectively. Although human IL-2 has only 60% amino acid homology with highly polymorphic mouse IL-2, human IL-2 is also biologically active on mouse cells. Cells reported to secrete IL-2 include γδ T cells, activated conventional CD4 + and CD8 + T cells, neurons, microglia, hematopoietic stem cells.
The IL-2 receptor (IL-2R) consists of three subunits, a 55 kDa CD25/IL-2Rα chain, a 70 kDa CD122/IL-2Rβ chain, and a 65 kDa CD132/γc chain. IL-2 first binds to CD25, and the two-subunit complex formed recruits CD122 and CD132, forming a signal complex with four subunits. In addition to forming a complex with IL-2, CD122/IL-2Rβ can also form a four-subunit signaling complex with IL-15. CD132/ Also acts as a receptor for IL-4, IL-7, IL-9, IL-15 and IL-21 signaling.
In vitro studies have shown that IL-2 plays an important role in T cell activation and expansion. In vivo, IL-2 is key to the development, maintenance, and function of regulatory T cells (Tregs), and regulatory T cells can provide protection against autoimmune diseases. In addition, IL-2 can also promote autoimmune inflammation in its target organs by regulating the expression of T cell trafficking genes and the production of Th2 cytokines. In CD8 + T cell subsets, IL-2 is a necessary key to obtaining an optimal first-order response and differentiation into terminal effector cells. IL-2 also promotes the development and activation of CD8 + T cells into memory cells.
General Notes 1. Please use the kit within the validity period.
2. The components of different kits and different batch kits cannot be mixed.
3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step.
4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc.
5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it.
6. For scientific research only, not for in vitro diagnosis.
Storage Temp. Kit unopened, stored at 2-8 ℃.
Test Range 2.7pg/mL-2000pg/mL

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