TMED10 Recombinant Rabbit mAb (S-5197-25)

Catalog Number: S0B60805 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	TMED10
Synonyms	Transmembrane emp24 domain-containing protein 10; 21 kDa transmembrane-trafficking protein; S31I125; S31III125; Tmp-21-I; Transmembrane protein Tmp21; TMP21
Immunogen	Synthetic Peptide
Location	Cell membrane, Endoplasmic reticulum
Accession	P49755
Clone Number	S-5197-25
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	U-87 MG, BxPC-3, Raji, 293T, HeLa, K-562, RAW264.7, NIH/3T3, Neuro-2a, mouse pancreas, mouse liver, mouse brain, PC-12, rat pancreas, rat brain, rat liver
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:10000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

TMED10 (transmembrane emp24 domain-containing protein 10, also known as Tmp21 or p24δ1) is a 219-amino-acid single-pass transmembrane protein encoded by the TMED10 gene in the q24.3 region of human chromosome 14, a member of the p24 family, and structurally contains a luminal signal peptide, a GOLD domain, a coiled-coil domain, a transmembrane region, and a cytoplasm-facing C-terminal tail. Its classical function is to serve as a cargo receptor in vesicular transport between the endoplasmic reticulum and the Golgi apparatus, forming hetero-oligomers with p24 family members such as TMED2 through its GOLD domain and mediating the sorting and transport of GPI-anchored proteins and secretory cargo in COPI- and COPII-coated vesicles. Recent studies have revealed another key role of TMED10: it can act as a protein channel mediating unconventional protein secretion (UPS), facilitating the entry of mature IL-1β and other signal peptide-lacking cargo proteins into the ERGIC with the assistance of the unfolded chaperone HSP90; this process depends on direct interaction between the C-terminal tail of TMED10 and cargo recognition motifs, and TMED10 oligomerization is induced by the presence of UPS cargo. In addition, TMED10 also differentially regulates Aβ40 production as a component of the γ-secretase complex, and its downregulated expression in Alzheimer's disease can activate ATG4B-dependent autophagy.

Picture

Western Blot

WB result of TMED10 Recombinant Rabbit mAb
Primary antibody: TMED10 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 μg
Lane 2: BxPC-3 whole cell lysate 20 μg
Lane 3: Raji whole cell lysate 20 μg
Lane 4: 293T whole cell lysate 20 μg
Lane 5: HeLa whole cell lysate 20 μg
Lane 6: K-562 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 19 kDa

WB result of TMED10 Recombinant Rabbit mAb
Primary antibody: TMED10 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 μg
Lane 2: NIH/3T3 whole cell lysate 20 μg
Lane 3: Neuro-2a whole cell lysate 20 μg
Lane 4: mouse pancreas lysate 20 μg
Lane 5: mouse brain lysate 20 μg
Lane 6: mouse liver lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 19 kDa

WB result of TMED10 Recombinant Rabbit mAb
Primary antibody: TMED10 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 μg
Lane 2: rat pancreas lysate 20 μg
Lane 3: rat brain lysate 20 μg
Lane 4: rat liver lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 19 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-TMED10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.