WB result of TIM Rabbit pAb
Primary antibody: TIM Rabbit pAb at 1/1000 dilution
Lane 1: HEK 293 whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 139kDa
TIM Rabbit Polyclonal Antibody
TIM Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TIM |
| Synonyms | Protein timeless homolog; TIM1; TIMELESS1; TIMELESS |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9UNS1 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HEK293, JAR, Raji, Jurkat, HeLa, RAW264.7, NIH/3T3, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| IHC-P | 1:200 | Hu |
Background
Protein timeless homolog (TIMELESS) is a highly conserved multifunctional nuclear protein in mammals, with the human gene located at chromosome 12q13.3 and encoding a protein of approximately 1208 amino acids. Unlike Timeless in Drosophila, which serves as a core circadian clock component, mammalian TIMELESS has shifted its primary function toward genome maintenance: it forms a heterodimer with TIPIN and acts as a key component of the replication fork protection complex, playing a central role in DNA replication and replication stress responses. This complex localizes to replication forks by binding replication protein A (RPA), inhibits the ATPase activity of the CMG helicase, and activates DNA polymerase, thereby coordinating the coupling of DNA unwinding and synthesis. In replication checkpoint signaling, the TIMELESS-TIPIN complex promotes nuclear accumulation of CLASPIN and activation of CHK1, participating in the response to replication stress induced by hydroxyurea or ultraviolet radiation. In addition, TIMELESS is involved in maintaining telomere length, promoting homologous recombination repair, stabilizing continuous DNA synthesis past G-quadruplex regions, and protecting genomic stability during cellular senescence. Although its dominant function in mammals is replication fork-related, TIMELESS still retains some circadian regulatory capacity, interacting with PER proteins and negatively regulating CLOCK/BMAL1-mediated transcriptional activation of PER1. Abnormal expression of this protein is associated with various diseases, including prostate cancer, lung cancer, breast cancer, and psychiatric disorders.
Picture
Picture
Western Blot
WB result of TIM Rabbit pAb
Primary antibody: TIM Rabbit pAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 139kDa
WB result of TIM Rabbit pAb
Primary antibody: TIM Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 139kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-TIM antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TIM antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TIM antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-TIM antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
