2μg (R: reducing condition, N: non-reducing condition).
IL-15RA His Tag Protein, Mouse
IL-15RA His Tag Protein, Mouse
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$180 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | CD215,IL-15R-alpha |
| Accession | Q60819 |
| Amino Acid Sequence | Gly33-Lys205 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 55-70kDa (Reducing) |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Huang YS, Cheng SN, Chueh SH, Tsai YL, Liou NH, Guo YW, Liao MH, Shen LH, Chen CC, Liu JC, Ma KH. Effects of interleukin-15 on neuronal differentiation of neural stem cells. Brain Res. 2009 Dec 22;1304:38-48. 2.Marzetti E, Carter CS, Wohlgemuth SE, Lees HA, Giovannini S, Anderson B, Quinn LS, Leeuwenburgh C. Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction. Mech Ageing Dev. 2009 Apr;130(4):272-80. |
Background
Interleukin-15 receptor alpha subunit (IL-15Rα, also known as CD215) is a widely expressed 60 kDa transmembrane glycoprotein that serves as a high-affinity binding protein for IL-15 and is crucial for memory CD8 T cell proliferation as well as the development of NK cells, NK/T cells, and intestinal intraepithelial lymphocytes. Structurally, IL-15R forms a heterotrimeric receptor complex with IL2RB and IL2RG and can signal in both cis and trans manners (i.e., IL-15R on one cell subset presents IL-15 to neighboring IL2RG-expressing cells); expression of different isoforms may alter or interfere with signal transduction, and its signaling involves SYK. Functionally, this receptor mediates multiple immunoregulatory functions of IL-15, and soluble sIL-15Rα levels are higher than membrane-bound forms in all brain structures. Clinically, the IL-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells and has shown significant anti-tumor effects.
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SDS-PAGE
SEC-HPLC
The purity of IL-15RA His Tag Protein, Mouse is more than 95% as determined by SEC-HPLC.
ELISA
Immobilized IL-15 Protein, Mouse (Cat. No. UA040513) at 5 μg/mL (100 μL/well) can bind IL-15RA His Tag Protein, Mouse(Cat. No. UA016255) with a linear range of 23-28 ng/mL.
