2μg (R: reducing condition, N: non-reducing condition).
IL-15RA His Tag Protein, Human
IL-15RA His Tag Protein, Human
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$345 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD215, IL15RA, IL-15R-alpha |
| Accession | Q13261 |
| Amino Acid Sequence | IIe31-Thr205with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 55-60kDa (Reducing) |
| Endotoxin | <0.1EU/μg |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt,lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃under sterile conditions after reconstitution. · 1 week, 2 to 8℃under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Amaria R N, Chesney J A, Betof A S, et al. Regulatable membrane-bound IL-15 engineered TIL cell therapy for immune checkpoint inhibitor-resistant advanced melanoma: pooled analysis of phase 1 and 1/2 trials[J]. Nature Medicine, 2026. 2. Rafei H, Basar R, Acharya S, et al. CREM is a regulatory checkpoint of CAR and IL-15 signalling in NK cells[J]. Nature, 2025, 643(8073): 1076-1086. 3. Choudhary N, Faiyazuddin M, Kamal R, et al. IL-15 superagonists for reprogramming the immunosuppressive microenvironment in gastrointestinal adenocarcinomas[J]. Cancer Letters, 2026, 659: 218780. |
Background
Interleukin-15 receptor α (IL-15Rα), also known as CD215, is the private, high-affinity receptor subunit for IL-15. It is a type I transmembrane glycoprotein whose extracellular region contains a single sushi domain that binds IL-15 with picomolar affinity. When co-expressed with IL-15, IL-15Rα forms an IL-15/IL-15Rα complex that is displayed on the cell surface and trans-presented to neighboring cells expressing the IL-2Rβ/γc heterodimer; metalloprotease cleavage additionally generates soluble IL-15Rα. IL-15Rα is expressed mainly by dendritic cells, monocytes/macrophages, and stromal cells. It does not signal directly; rather, it binds, stabilizes, traffics, and presents IL-15, while signal transduction through IL-2Rβ/γc activates JAK1/JAK3–STAT5, PI3K-AKT, and MAPK pathways that drive the development, proliferation, survival, and cytotoxic function of NK cells and memory CD8⁺ T cells. Clinically, IL-15/IL-15Rα complexes and the IL-15 superagonist N-803 (nogapendekin alfa inbakicept) have been approved for BCG-unresponsive non-muscle-invasive bladder cancer, and IL-15Rα-based constructs are widely explored in armored CAR-NK/CAR-T cells, TIL therapy, chronic infections, and autoimmune disease.
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SDS-PAGE
SEC-HPLC
The purity of IL-15RA His Tag Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized Human IL-15 Protein, hFc tag (Cat. No. S0A4044) at 5 μg/mL (100 μL/well) can bind IL-15RA His Tag Protein, Human (Cat. No. UA016258) with a linear range of 1.6-1.9 ng/mL.
