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Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb (S-4912-35)

Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb (S-4912-35)

Catalog Number: S0B60737 Application: WB Reactivity: Hu Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Cleaved Caspase-8 (Asp374)
Synonyms Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE (FLICE); MCH5; CASP8
Immunogen Synthetic Peptide
Location Cytoplasm, Nucleus
Accession Q14790
Clone Number S-4912-35
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Hu
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu

Background

Cleaved Caspase-8 (Asp374) is a key protein involved in the extrinsic apoptosis pathway, which is activated by death receptors such as Fas (CD95) and tumor necrosis factor receptor 1 (TNFR1). When cleaved at Asp374, it generates active fragments, including the p18 subunit, which can further activate downstream effector caspases like caspase-3, -6, and -7, ultimately leading to apoptosis. This protein is also implicated in regulating inflammatory necrotic cell death pathways such as necroptosis and pyroptosis.

Picture

Western Blot

WB result of Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-8 (Asp374) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 μg
Lane 2: Jurkat treated with 1μM staurosporine for 4 hours whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Observed MW: 18, 41, 43 kDa