Flow cytometric analysis of human peripheral blood cells labeling Biotin Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) followed by Sav-iFluor 488 compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse IgG2b, κ Isotype Control (S-5677)
Biotin Mouse IgG2b, κ Isotype Control (S-5677)
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$120 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-5677 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) labeling Biotin Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) followed by Sav-iFluor 488 compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized human PBMCs (human peripheral blood mononuclear cells) labelling Biotin Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) followed by Sav-iFluor 488 compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized 293F (Human embryonic kidney epithelial cell) labelling Biotin Mouse IgG2b, κ Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) followed by Sav-iFluor 488 compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
