Annexin-4/ANXA4 Recombinant Rabbit mAb (S-6264)

Catalog Number: S0B60713 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Annexin-4/ANXA4
Synonyms	Annexin A4; 35-beta calcimedin; Annexin IV; Carbohydrate-binding protein p33/p41; Chromobindin-4; ANX4
Location	Cytoplasm
Accession	P09525
Clone Number	S-6264
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, Hep G2, RAW264.7, NIH/3T3, mouse liver, mouse kidney, mouse lung, C6, PC-12, rat liver, rat kidney, rat lung
Purification	Protein A
Concentration	0.25 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:2500	Hu, Ms, Rt
ICC	1:250	Hu

Background

Annexin A4 (ANXA4), also known as lipocortin IV or 36 kDa zymogen granule membrane-associated protein (ZAP36), is a calcium- and phospholipid-binding protein belonging to the annexin superfamily, encoded by the ANXA4 gene. Structurally, it contains a conserved C-terminal core domain composed of four homologous annexin repeats that mediate calcium-dependent phospholipid binding, and a short variable N-terminal domain that is subject to post-translational modifications such as phosphorylation and is thought to confer functional specificity. Annexin A4 is predominantly expressed in epithelial cells and is also found in secretory tissues including the pancreas, liver, intestine, and kidney. Functionally, it promotes calcium-dependent membrane aggregation and fusion, participating in exocytosis and membrane repair, and its lipid association is modulated by sodium ions, which induce dissociation from membranes. Beyond its physiological roles, Annexin A4 is overexpressed in numerous malignancies—including renal cell carcinoma, malignant mesothelioma, endometrial carcinoma, and cholangiocarcinoma—where it contributes to tumor progression and chemoresistance, particularly to cisplatin; notably, its expression is increased in cisplatin-resistant mesothelioma cells, and knockdown of ANXA4 restores drug sensitivity, supporting its potential as a biomarker and therapeutic target.

Picture

Western Blot

WB result of Annexin-4/ANXA4 Recombinant Rabbit mAb
Primary antibody: Annexin-4/ANXA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: Hep G2 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 31 kDa

WB result of Annexin-4/ANXA4 Recombinant Rabbit mAb
Primary antibody: Annexin-4/ANXA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 μg
Lane 2: NIH/3T3 whole cell lysate 20 μg
Lane 3: mouse liver lysate 20 μg
Lane 4: mouse kidney lysate 20 μg
Lane 5: mouse lung lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 31 kDa

WB result of Annexin-4/ANXA4 Recombinant Rabbit mAb
Primary antibody: Annexin-4/ANXA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 μg
Lane 2: PC-12 whole cell lysate 20 μg
Lane 3: rat liver lysate 20 μg
Lane 4: rat kidney lysate 20 μg
Lane 5: rat lung lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 31 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squmouas cell carcinoma. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-Annexin-4/ANXA4 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in Hep G2 cells. Anti- Annexin-4/ANXA4 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).