Research and Application Progress of SHPS1 Recombinant Protein
06 May 2026
by AntBio
Research and Application Progress of SHPS1 Recombinant Protein
Published by ANT BIO PTE. LTD.
1. Concept
SHPS1 (also known as SIRPα) is a highly glycosylated transmembrane immune regulatory protein that acts as a key receptor in the CD47–SHPS1 signaling axis. It modulates macrophage phagocytosis, immune tolerance, cell adhesion, and synaptic plasticity. SHPS1 recombinant protein refers to the bioactive form of SHPS1 produced via genetic engineering in heterologous expression systems, widely used for molecular interaction analysis, functional validation, drug screening, and diagnostic development.
2. Research Frontiers
Recent advances in SHPS1 recombinant protein technology and applications include:
- High-efficiency expression in mammalian, insect, and bacterial systems with native‑like post‑translational modifications.
- Engineered variants (phosphorylation mutants, glycosylation‑optimized forms, truncated ectodomains) for tailored research use.
- High‑resolution structural elucidation of the SHPS1–CD47 complex using cryo‑EM and X‑ray crystallography.
- Development of SHPS1‑based therapeutics, including blocking antibodies, fusion proteins, and small‑molecule modulators targeting the “don’t eat me” pathway.
- SHPS1 as a potential biomarker for cancer, neurodegeneration, and autoimmune diseases.
3. Research Significance
SHPS1 recombinant protein is indispensable for advancing immunology, oncology, and neuroscience:
- Enables mechanistic dissection of the CD47–SHPS1 immune checkpoint axis.
- Supports high‑throughput screening for cancer immunotherapy agents.
- Facilitates structural biology and drug design targeting immune regulation.
- Provides a standardized tool for diagnostic assay development and clinical biomarker detection.
4. Preparation, Quality Control, and Applications
4.1 Preparation Technologies
SHPS1 recombinant protein is commonly produced in three expression systems:
- Mammalian cells (HEK293, CHO): Supports authentic glycosylation and folding, ideal for full‑length functional protein.
- Insect cells (Sf9): Balances yield and post‑translational modification for structural studies.
- Prokaryotic cells (E. coli): Cost‑effective for producing non‑glycosylated functional domains.
Key steps include vector design with His/FLAG/Fc tags, transfection, stable cell line screening, detergent‑based solubilization, and multi‑step chromatography purification.
4.2 Quality Control and Characterization
Rigorous QC ensures reliable performance:
- Purity: ≥95% by SDS‑PAGE and HPLC.
- Molecular weight: Confirmed by mass spectrometry.
- Structure: Validated by CD, NMR, X‑ray crystallography, or cryo‑EM.
- Bioactivity: Measured by SPR/BLI for CD47 binding, in vitro phosphorylation, or phagocytosis assays.
- Stability: Evaluated for thermal, storage, and freeze–thaw tolerance; recommended storage at −80°C.
4.3 Research Applications
SHPS1 recombinant protein supports diverse studies:
- Molecular interactions: Quantifies SHPS1–CD47 binding affinity (nanomolar range).
- Signaling pathways: Activates SHP‑1/SHP‑2 and integrin signaling in a controlled manner.
- Drug discovery: Serves as a target for screening small molecules and therapeutic antibodies.
- Immunotherapy: Blocks the CD47 “don’t eat me” signal to enhance tumor clearance.
- Diagnostics: Used as a standard antigen for biomarker detection in serum or tissues.
[Image Location: Analytical profile of SHPS1 recombinant protein (e.g., SDS‑PAGE, HPLC, or binding kinetics curve)]
4.4 Challenges and Future Directions
Current limitations include delivery efficiency, production cost, and stability of full‑length membrane protein. Future directions focus on:
- Engineered SHPS1 variants with improved stability and affinity.
- Multifunctional fusion proteins for targeted therapy and imaging.
- Glycoengineering to optimize pharmacokinetics.
- Clinical translation as a therapeutic protein and diagnostic reagent.
5. Our Mission at ANT BIO PTE. LTD.
ANT BIO PTE. LTD. is dedicated to accelerating breakthroughs in immunology, oncology, and drug development by providing premium, bioactive SHPS1 recombinant proteins and supporting reagents. Our products are engineered for consistency, high purity, and reliable performance in basic research, drug screening, and translational applications.
6. Featured SHPS1‑Related Products
| Product Category | Brand | Key Applications |
|---|---|---|
| Recombinant Human SHPS1 Protein | UA | SPR/BLI binding assay, drug screening, functional study |
| Recombinant Mouse SHPS1 Protein | UA | Animal model research, in vitro activity assays |
| Anti‑SHPS1 Monoclonal Antibody | STARTER | WB, IHC‑P, ICC, IF, flow cytometry |
| SHPS1 ELISA Kit | Absin | Quantitative detection in cell lysate, tissue, plasma |
| Anti‑CD47 Antibody | STARTER | SHPS1–CD47 axis research |
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
