Recombinant P84 Protein: Structure, Function, and Translational Applications
Recombinant P84 Protein: Structure, Function, and Translational Applications – Supported by ANT BIO PTE. LTD.
Concept
P84 protein, also referred to as SHPS‑1 or SIRPα, is an 84 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. It features three extracellular Ig‑like domains, a transmembrane region, and an intracellular tail containing immunoreceptor tyrosine‑based inhibitory motifs (ITIMs). As a key regulator of immune tolerance, phagocytosis, and neural homeostasis, recombinant P84 has become a pivotal tool in oncology, neurology, and immunotherapy research.
Research Frontier
Recombinant P84 protein has rapidly emerged as a high‑impact target in translational medicine. Recent advances highlight its critical role in the CD47‑P84 “don’t eat me” signaling axis, which mediates tumor immune evasion. Meanwhile, engineered P84‑Fc fusion proteins show robust efficacy in preclinical models of cancer, neurodegeneration, and cerebrovascular injury. Innovations in expression systems, purification, and formulation have further accelerated its transition from basic research to clinical development.
Research Significance
P84 governs fundamental processes including macrophage phagocytosis, synaptic plasticity, myelin maintenance, and inflammatory regulation. Dysregulation of P84‑CD47 signaling contributes to tumor progression, neurodegeneration, and autoimmune disorders. Recombinant P84 serves as both a research probe for dissecting these pathways and a therapeutic candidate, offering new strategies for unmet medical needs in oncology and neurology.
Molecular Mechanisms & Research Methods
Structure & Expression
Native‑like recombinant P84 is primarily produced in mammalian cell lines (HEK293, CHO) to ensure authentic glycosylation and folding. Optimized vectors with signal peptides and affinity tags enable high yields (20–30 mg/L) and purity above 95%.
Interaction Mechanism
P84 binds specifically to CD47 via its N‑terminal IgV domain, triggering ITIM phosphorylation and recruitment of SHP‑1/SHP‑2 phosphatases. This pathway suppresses phagocytosis in myeloid cells and modulates neural development.
Functional Applications
- Tumor immunotherapy: P84‑Fc acts as a decoy receptor to block CD47, restoring macrophage‑mediated tumor clearance.
- Neuroprotection: Recombinant P84 reduces amyloid plaque burden, enhances myelin preservation, and limits ischemic injury.
- Functional studies: Common assays include SPR, CD, DLS, Western blot, immunofluorescence, and cell‑based phagocytosis tests.
How ANT BIO PTE. LTD. Empowers P84 Research
ANT BIO PTE. LTD. delivers premium reagents to support every stage of P84‑related research:
- Recombinant Proteins (UA sub‑brand): High‑purity, bioactive recombinant P84, CD47, SHP‑1, and SHP‑2 proteins optimized for binding, functional, and cellular assays.
- Antibodies (STARTER sub‑brand): Validated antibodies against P84, CD47, phospho‑ITIM, and macrophage activation markers for Western blot, IHC, IF, and flow cytometry.
- Kits & Reagents (Absin sub‑brand): ELISA kits for quantifying P84/CD47, protein purification resins, stability enhancers, and cell lysis buffers.
Our products ensure reliable, reproducible results for protein characterization, molecular interaction, and therapeutic development.
Brand Mission
ANT BIO PTE. LTD. is committed to advancing global life science discovery and translational medicine by providing high‑quality, innovative reagents and integrated solutions. We empower researchers to accelerate mechanistic studies, drug development, and clinical translation.
Related Product Portfolio
- Recombinant P84 / SIRPα / SHPS‑1 protein
- Recombinant CD47 protein
- Anti‑P84 / anti‑CD47 antibodies
- P84‑CD47 binding ELISA kits
- Protein purification & stabilization reagents
- Immunology and neurology research assay kits
Brand Promotion
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
