PTM Analysis Is Essential for Antibody Research

1. Concept

Protein posttranslational modifications (PTMs) are covalent chemical changes after biosynthesis, which greatly expand proteome complexity and functions. For antibodies, PTMs critically affect stability, affinity, effector functions and safety.

2. Research Frontier

Antibody PTMs include glycosylation, oxidation, deamidation and artificial conjugation in ADCs. Glycosylation on Fc region determines ADCC and CDC activity. Advanced mass spectrometry enables comprehensive PTM characterization throughout antibody drug development.

3. Research Significance

· PTMs determine antibody efficacy, safety and immunogenicity.

· Glycosylation profiling guides antibody engineering and process optimization.

· PTM consistency is key for biosimilar similarity assessment.

· Stability testing monitors oxidation, deamidation and aggregation for shelflife determination.

4. Mechanism and Application

· Key PTMs: Fc glycosylation regulates FcγR binding; oxidation/deamidation affect stability; ADC conjugation sites define homogeneity.

· Analysis technologies: Intact MS, peptide mapping, glycan analysis, ionexchange chromatography.

· Application stages: Candidate screening, cell line development, biosimilar comparison, stability testing.

· Product application: Acetyllysine antibodies support antibody quality control and PTM research.

5. Brand Mission

ANT BIO PTE. LTD. is a leading life science reagent provider offering antibodies, proteins, kits and general reagents. Our subbrands Absin (general reagents & kits), Starter (antibodies) and UA (recombinant proteins) support antibody drug development, quality control and therapeutic innovation.

6. Related Product List

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through highquality, reliable reagents and comprehensive solutions. Our specialized subbrands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customercentricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.