Applications and Quality Control Strategies of CD117 Antibody in Immunohistochemical Detection
Knowledge Insight: Applications and Quality Control Strategies of CD117 Antibody in Immunohistochemical Detection
Concept
CD117 (c‑Kit) is a type III receptor tyrosine kinase encoded by the c‑kit proto‑oncogene. It acts as the transmembrane receptor for stem cell factor (SCF) and regulates cell proliferation, differentiation, and survival. In clinical pathology, CD117 is a definitive diagnostic marker for gastrointestinal stromal tumors (GISTs) and is also expressed in mast cells, germ cell tumors, melanocytes, and interstitial cells of Cajal.
Research Frontier
Modern CD117 IHC detection emphasizes standardized reagents, quantitative quality control, and multi‑marker panels. Emerging innovations include liquid‑based cell controls, digital pathology integration, AI‑assisted interpretation, and combination testing with DOG1 and CD34 to improve diagnostic accuracy for GIST and other spindle cell neoplasms.
Research Significance
CD117 IHC is indispensable in surgical pathology because it:
- Serves as the gold‑standard marker for GIST diagnosis
- Enables differential diagnosis of spindle cell tumors
- Supports classification of germ cell tumors and mast cell diseases
- Guides targeted therapy selection and prognostic evaluation
- Requires rigorous quality control to ensure clinical reliability
Core Mechanisms and Technical Methods
Biological Features of CD117
- Expressed in normal mast cells, melanocytes, and interstitial cells of Cajal
- Shows membranous and cytoplasmic staining in positive cells
- Pathologically activated in GISTs, mastocytosis, and certain germ cell tumors
Standardized IHC Requirements
- Use regulatory‑compliant, well‑validated CD117 antibodies
- Combine with DOG1, CD34, and other markers for enhanced specificity
- Include mandatory positive and negative tissue/cell controls
Liquid Cell Control Materials
Liquid‑based cell controls represent an advanced QC tool with these benefits:
- Stable, consistent positive/negative staining patterns
- Easy to use, ready‑to‑apply format
- Solves scarcity of rare positive control tissues
- Compatible with digital pathology and AI analysis
- Enables slide‑level internal quality control
Comprehensive Quality Control System
- Daily internal QC with liquid cell controls
- Regular external proficiency testing
- Multi‑marker diagnostic panels
- Standardized operating procedures (SOPs)
- Full‑process pre‑analytical to post‑analytical monitoring
Future Development Directions
- Next‑generation liquid‑based QC materials
- AI‑assisted digital IHC interpretation
- Multi‑omics integration with genetic sequencing
- Systemic full‑cycle quality management
Product Empowerment
ANT BIO PTE. LTD. provides the S‑RMab® CD117 Recombinant Rabbit Monoclonal Antibody (SDT‑125‑51, Cat. No. S0B2084) under its Starter product line. This high‑performance reagent delivers exceptional specificity, clean membrane/cytoplasmic staining, and excellent batch consistency for diagnostic‑grade IHC testing.
It is validated for FFPE tissues and supports GIST diagnosis, mast cell disease identification, germ cell tumor typing, and stem cell research.
Core Product Advantages
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Core Product Advantages |
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High Specificity & Precise Localization: Specifically targets human CD117/c‑Kit; clear, distinct membranous and cytoplasmic staining in FFPE sections with clean background. |
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Excellent Stability & Batch Consistency: Strict QC ensures minimal inter‑batch variation and reliable performance for clinical diagnostics and multi‑center studies. |
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Diagnostic‑Grade Performance: Optimized for pathological IHC; supports accurate tumor subtyping and differential diagnosis. |
Key Application Scenarios
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Key Application Scenarios |
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GIST Diagnosis: Core marker for identifying gastrointestinal stromal tumors and differentiating from other spindle cell neoplasms. |
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Mast Cell Disease: Diagnosis of systemic mastocytosis, mastocytoma, and related disorders. |
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Germ Cell Tumors: Detection of seminoma, dysgerminoma, and other germ cell malignancies. |
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Stem Cell Research: Identification and tracking of hematopoietic stem cells and melanocyte lineage cells. |
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Pathology QC & Validation: Supported by standardized controls for reliable clinical testing. |
Brand Mission
ANT BIO PTE. LTD. is a leading provider of life science reagents, offering antibodies, recombinant proteins, assay kits, and general laboratory reagents. We operate three specialized sub‑brands: Absin (general reagents & kits), Starter (antibodies), and UA (recombinant proteins), delivering integrated solutions for diagnostic pathology, oncology, and translational medicine. We uphold strict quality standards to support accurate, reproducible, and clinically meaningful IHC testing.
Related Product List
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Product Name |
Catalog No. |
Applications |
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CD117 Recombinant Rabbit Monoclonal Antibody (SDT‑125‑51) |
S0B2084 |
IHC‑FFPE, GIST diagnosis, tumor typing |
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GIST Diagnostic Antibody Panel (CD117/DOG1/CD34) |
Starter series |
Combined differential diagnosis |
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IHC Liquid Cell Positive/Negative Controls |
Absin series |
Daily quality assurance |
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IHC Detection Kits & Buffers |
Absin series |
Standardized staining systems |
Brand Promotion Copy
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
