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Human GH Protein, His Tag

Human GH Protein, His Tag

Catalog Number: S0A8004 Brand: Starter
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$100 USD
$100 USD
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Product Details

Product Specification


Species Human
Synonyms Somatotropin; Growth hormone 1; Pituitary growth hormone
Accession P01241
Amino Acid Sequence

Protein sequence (P01241, Phe27-Phe217, with C-His Tag) FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
Expression System E.coli
Molecular Weight Predicted MW: 23.9 kDaObserved MW: 24 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Growth hormone (GH) or somatotropin, also known as human growth hormone (hGH or HGH) in its human form, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH also stimulates production of IGF-1 and increases the concentration of glucose and free fatty acids. It is a type of mitogen which is specific only to the receptors on certain types of cells. GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland.

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Bioactivity

Immobilized Human GH Protein, His Tag at 2.0μg/mL (100μL/well) can bind GHR Fc Chimera Protein, Mouse (Cat. No. UA011366) with EC50 of 1.44-2.12 ng/mL.

SDS-PAGE

2μg（R: reducing conditions）