Flow cytometric analysis of CAR (FMC63 scFv) transfected 293T (Human embryonic kidney epithelial cell (right panel) or untrasfected 293T (left panel) labeled FMC63 antibody at 1/2000 (0.1 μg) dilution. Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FMC63 Recombinant Rabbit mAb (S-2163-77)
FMC63 Recombinant Rabbit mAb (S-2163-77)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FMC63 scFv |
| Immunogen | Recombinant Protein |
| Clone Number | S-2163-77 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B0248 |
| Application | FCM |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Species independent |
Background
FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.
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