Standard Curve
Example of Monkey IL-2 standard curve in Assay Diluent DE4. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Monkey IL-2 OneStep ELISA Kit
Monkey IL-2 OneStep ELISA Kit
Price:
Regular price
$667 USD
Regular price
Sale price
$667 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IL-2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 4.1%； Inter-assay: 4.4% |
| Sample type | Serum; EDTA Plasma, Citrate Plasma; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.07 pg/mL |
| Range | 3.91 pg/mL – 250 pg/mL |
| Recovery | Serum: 110% EDTA Plasma: 102% Citrate plasma: 110% Cell Culture Supernatant: 103% |
| Assay time | 60 minutes |
| Species reactivity | Mk |
| CROSS REACTIVITY | Hu |
Background
Monkey interleukin-2 (IL-2) is a 134-amino-acid glycoprotein cytokine (~15.5 kDa) that shares extensive structural and functional homology with human IL-2, exhibiting approximately 96% amino acid sequence identity and cross-reactivity with anti-human IL-2 antibodies. It is a cytokine that plays a key role in regulating immune responses, particularly in T-cell proliferation. It signals through a heterotrimeric receptor complex (IL-2Rα/β/γ) to drive T cell proliferation, enhance NK and B cell activity, and coordinate adaptive immune responses. Beyond basic immunology, it is indispensable in preclinical non-human primate models particularly Rhesus Macaques for studying HIV/SIV pathogenesis, evaluating novel IL-2-based immunotherapies (including tumor-selective masked constructs), and assessing the safety, pharmacokinetics, and translational potential of next-generation biologics before clinical application.
Picture
Picture
ELISA
Spike Recovery
The recovery of Monkey IL-2 was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Dilution linearity
The concentrations of Monkey IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as 1% neat biological sample. The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 38140.2 pg/mL in Monkey PBMC stimulated supernatant.
Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Monkey IL-2 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross-reactivity
The cross-reactivity between Monkey IL-2, Human IL-2, Mouse IL-2 and Rat IL-2 was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Mouse IL-2 or Rat IL-2 was tested in the Monkey IL-2 assay, as the signals remained within the background range of the assay.
Significant cross-reactivity was observed when Human IL-2 was tested in the Monkey IL-2 assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
