Skip to product information
1 of 3

Human IL-5 OneStep ELISA Kit

Human IL-5 OneStep ELISA Kit

Catalog Number: S0C3009 Application: ELISA Reactivity: Human Conjugation: Brand: Starter
Price:
Regular price $500 USD
Regular price Sale price $500 USD
Size:
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Antigen IL-5
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

2 to 8 °C as supplied.

Kit


Precision

Intra-assay: 4.7%;

Inter-assay: 6.4%

Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 1.1 pg/ml
Range 3.9 pg/ml - 250 pg/ml
Recovery Cell culture supernatant 85%
Serum 102%
Plasma 97%
Assay time 60 minutes
Species reactivity Human
CROSS REACTIVITY /
INTERFERENCE /
NIBSC convert

The dose response curve of the International Standard (90/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (90/586) units, use the following equation: NIBSC (90/586) approximate value (IU/mL) = 0.01 x OneStep Human IL-5 value (pg/mL).

Background

IL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.

Picture

ELISA

Standard curve
Example of human IL-5 standard curve in Assay Diluent #1.

Standard curve
Example of human IL-5 standard curve in Assay Diluent #1S5.

Linearity
The concentrations of IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is  undiluted samples are as follows:  human PBMC cells stimulated with 10ug/ml PHA for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 167 pg/mL in stimulated human PBMC  supernatant.
#2 sample is  undiluted samples are as follows:  human PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 5 pg/mL in unstimulated human PBMC  supernatant.

Leading Competitor comparison

Protocol Diagram

Standard curve
Example of human IL-5 standard curve in Assay Diluent #1.

Customer Reviews

Be the first to write a review
0%
(0)
0%
(0)
0%
(0)
0%
(0)
0%
(0)