Protein G Donor Beads

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads, resulting in luminescence.

Donor beads recognize Protein 1 (Tag1 label), and Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requires no washing, and offers high speed and sensitivity. It can detect weak binding interactions.

1. This experiment is light-sensitive; take care to avoid light during operation. It is recommended to perform the preparation, sample addition, and incubation steps under green light conditions (illuminance below 100 LUX).

2. This product is compatible with multi-mode microplate readers equipped with an Alpha detection module.

3. Before use, vortex thoroughly to mix; a brief centrifugation (2000×g for 5–10 seconds) may also be performed to ensure complete recovery of the reagent.

4. We recommend using our company's dedicated dilution buffer for reagent preparation and analyte dilution; if special additives are required, they can be directly added to this dilution buffer.

5. To ensure comparability of experimental data across different batches, please strictly control the incubation temperature and time.

6. When adding samples, be careful to avoid introducing air bubbles.