Standard Curve
Example of Mouse IL-6 standard curve in Assay Diluent EC8. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Mouse IL-6 OneStep ELISA Kit
Mouse IL-6 OneStep ELISA Kit
Price:
Regular price
$120 USD
Regular price
Sale price
$120 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.8%； Inter-assay: 4.2% |
| Sample type | Serum (Balb/c); Heparin Plasma (CD-1), Citrate Plasma (Balb/c, C57BL/6); Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.87 pg/mL |
| Range | 7.8 pg/mL – 500 pg/mL |
| Recovery | Serum (Balb/c): 106% Citrate plasma (Balb/c):113% Citrate plasma (C57BL/6): 93% Heparin Plasma (CD-1): 106% Cell Culture Supernatant: 111% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse interleukin-6 (IL-6) is a pleiotropic cytokine encoded by the Il6 gene on chromosome 5, produced primarily by immune cells, fibroblasts, and endothelial cells in response to infection, injury, or inflammatory stimuli, and it exerts its biological effects through a receptor complex composed of IL-6Rα and gp130, activating downstream JAK/STAT3, MAPK, and PI3K signaling cascades that drive diverse functions including acute-phase protein synthesis in hepatocytes, Th17 cell differentiation, B-cell maturation, osteoclastogenesis, and metabolic regulation, while its dysregulation is linked to chronic inflammation and autoimmune pathology, as demonstrated by Il6-deficient mice which show reduced inflammatory responses and resistance to experimental autoimmune encephalomyelitis, highlighting its central role as both a protective mediator of host defense and a pathogenic driver in inflammatory disease models.
Picture
Picture
ELISA
Spike Recovery
The recovery of Mouse IL-6 was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
