Human IL-2 Kit (HICA)
Human IL-2 Kit (HICA)
Product Details
Product Details
Product Specification
| Stability & Storage | Store at 2~8°C protected from light for 18 months; after reconstitution, the standard solution can be aliquoted and stored at -20°C, avoiding repeated freeze-thaw cycles. |
Background
Test Principle:
This kit employs a homogeneous immuno chemiluminescence assay (HICA) based on the double-antibody sandwich method for the detection of cytokine concentrations. The operation is simple and requires no washing steps.
The detection system includes two types of microspheres: acceptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two microspheres into close proximity. When the distance between the donor and acceptor microspheres is less than 200 nm, singlet oxygen generated upon light excitation can transfer to the acceptor microsphere and trigger chemiluminescence. Conversely, if the target protein is absent, the distance between the microspheres is too large, resulting in no signal.
By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method offers advantages such as simplicity, rapid reaction, and high sensitivity.
Components
Name |
Specification |
Component Specification |
hIL-2 Assay Reagent R1 |
100 Tests |
400 μL/bottle × 1 |
500 Tests |
2 mL/bottle × 1 |
|
2,000 Tests |
8 mL/bottle × 1 |
|
10,000 tests |
40 mL/bottle × 1 |
|
hIL-2 assay reagent R2 |
100 tests |
400 μL/bottle × 1 |
500 tests |
2 mL/bottle × 1 |
|
2,000 tests |
8 mL/bottle × 1 |
|
10,000 tests |
40 ml/bottle × 1 |
|
hIL-2 assay reagent R3 |
100 tests |
1 mL/bottle × 1 |
500 tests |
5 mL/bottle × 1 |
|
2,000 tests |
20 mL/bottle × 1 |
|
10,000 tests |
100 mL/bottle × 1 |
|
hIL-2 standard |
100 tests |
1.67 μg lyophilized powder × 1 |
500T |
1.67μg lyophilized product × 1 |
|
2000T |
1.67μg lyophilized product × 2 |
|
10000T |
1.67μg lyophilized product × 5 |
|
hIL-2 standard buffer |
100T |
1.2mL/bottle × 1 |
500T |
6 mL/bottle × 1 |
|
2000T |
12 mL/bottle × 1 |
|
10,000T |
30 mL/bottle × 1 |
Note: It is recommended to use microplates (384- or 96-well plates, white, with shallow wells) for this assay.
Guidelines
Reagent R3 must be protected from light. Sampling and incubation should be performed under green light (<100 LUX).
Recalibration is required for each test, with at least duplicate wells for each standard concentration point. A four-parameter (weighting: 1/Y²) fitting calculation should be applied.
Temperature and time must be controlled during incubation. Microplates should be covered with a film, and a microplate reader with ALPHA function is recommended.
The dilution matrix for calibrators should match the test samples. Reconstituted calibrators must be used within 2 hours.
Components from different reagent kit lots must not be mixed.
