Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) labeling Mouse IgG2c Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Product Details
Product Details
Product Specification
| Host | Mouse |
| Clone Number | S-4245 |
| Antibody Type | Mouse mAb |
| Application | In vivo control |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized human PBMC (human peripheral blood mononuclear cells) labelling mouse IgG2c Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
