WB result of Invivo anti-mouse IL-7Rα (CD127) Recombinant mAb
Primary antibody: Invivo anti-mouse IL-7Rα (CD127) Recombinant mAb at 1/1000 dilution
Lane 1: IL-7R alpha/CD127 Fc Chimera Protein, Mouse 2 µg
Lane 2: IL-7R alpha/CD127 Fc Chimera Protein, Human 2 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 60-90 kDa
Invivo anti-mouse IL-7Rα (CD127) Recombinant mAb
Invivo anti-mouse IL-7Rα (CD127) Recombinant mAb
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$150 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-7Rα (CD127) |
| Synonyms | Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; Il7r |
| Location | Membrane |
| Accession | P16872 |
| Antibody Type | Recombinant mAb |
| Isotype | Rat IgG2a,k |
| Application | in vivo blocking of IL-7Rα signaling |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Purity | >95% Determined by SEC-HPLC |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Interleukin-7 receptor α chain (IL-7Rα, CD127) is a 75 kDa type-I transmembrane glycoprotein that forms the cytokine-binding subunit of both the IL-7 receptor (IL-7R) and the thymic stromal lymphopoietin receptor (TSLPR); upon binding IL-7 it heterodimerizes with the common gamma chain (γc, CD132) to initiate JAK1/JAK3-mediated phosphorylation of STAT5, thereby driving survival, proliferation and differentiation of T, B and innate lymphoid cells, while TSLP engagement pairs IL-7Rα with the TSLPR chain to modulate type-2 immunity.
Picture
Picture
Western Blot
