WB result of Invivo anti-Mouse IL-6 Recombinant mAb
Primary antibody: Invivo anti-Mouse IL-6 Recombinant mAb at 1/1000 dilution
Lane 1: IL-6 Protein, Mouse 10 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
Invivo anti-Mouse IL-6 Recombinant mAb
Invivo anti-Mouse IL-6 Recombinant mAb
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$196.00 SGD
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$196.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-6 |
| Synonyms | Interleukin-6; B-cell hybridoma growth factor; Interleukin HP-1 |
| Location | Secreted |
| Accession | P08505 |
| Clone Number | S-R550 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | in vivo IL-6 neutralization, in vitro IL-6 neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | Lot specific* (generally 2 to 10 mg/ml)* |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
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Western Blot
