WB result of Invivo anti-Mouse IL-2 Recombinant mAb
Primary antibody: Invivo anti-Mouse IL-2 Recombinant mAb at 1/1000 dilution
Lane 1: IL-2 Protein, Mouse lysate 1 µg
Lane 2: IL-2 Protein, Rat lysate 1 µg
Lane 3: IL-2 Protein, Human lysate 1 µg
Secondary antibody: Goat Anti-Rat IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-2 |
| Synonyms | Interleukin-2; T-cell growth factor (TCGF); Il-2; Il2 |
| Location | Secreted |
| Accession | P04351 |
| Clone Number | S-5581 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2a, κ |
| Isotype Control | S0B0932 |
| Application | WB, in vivo neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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Western Blot
