Flow cytometric analysis of BMDM (Bone Marrow-Derived Macrophages) labelling F4/80 antibody at 1/500 dilution (1 μg) / (red) compared with a Rat IgG2b, κ (black) Isotype Control. Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo Anti-Mouse F4/80 Recombinant mAb
Invivo Anti-Mouse F4/80 Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | F4/80 |
| Synonyms | Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1 |
| Location | Cell membrane |
| Accession | Q61549 |
| Clone Number | S-2753 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2b,k |
| Application | FCM, Functional assays |
| Reactivity | Ms |
| Positive Sample | BMDM |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, Contains no stabilizers or preservatives |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
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