Animal species and strain: BALB/c
Cell line: EMT6
Invivo anti-mouse CTLA-4 (CD152) mAb
Invivo anti-mouse CTLA-4 (CD152) mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Synonyms | Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4 |
| Location | Cell membrane |
| Accession | P09793 |
| Clone Number | 9D9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | WB, in vivo CTLA-4 neutralizatior |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | Lot specific* (generally 5 to 20 mg/ml)* |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
This antibody recognizes the same epitope as clone 9D9.
Cytotoxic T-lymphocyte associated protein 4, (CTLA-4) also known as CD152 (cluster of differentiation 152), is a protein receptor that functions as an immune checkpoint and downregulates immune responses. CTLA-4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. CTLA-4 may also function via modulation of cell motility and/or signaling through PI3 kinase.
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Validation Data
Animal species and strain: BALB/c
Cell line: EMT6
Western Blot
WB result of Invivo CTLA-4 (CD152) Mouse mAb
Primary antibody: Invivo CTLA-4 (CD152) Mouse mAb at 1/1000 dilution
Lane 1: reduced purified mouse CTLA-4 with histidine tag at C-terminus 1 µg
Lane 2: reduced purified mouse CTLA-4 with histidine tag at C-terminus 0.5 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25~30 kDa
Observed MW: 20~30 kDa
SPR
Anti-His antibody Immobilized on CM5 Chip captured CD152 His Tag, Mouse, can bind CTLA-4 (CD152) Mouse mAb (9D9) with an affinity constant of 2.315 nM as determined in SPR assay.
