Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD3ε at 1/50000 (0.01 μg) dilution / (Right panel) compared with a Mouse IgG, Isotype Control / (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.
Product Details
Product Details
Product Specification
| Host | Mouse |
| Synonyms | T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e |
| Location | Cell membrane |
| Accession | P22646 |
| Clone Number | 145-2C11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1,k |
| Application | FCM, in vitro T cell stimulation/activation, in vivo T cell depletion |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| FCM | 1:50000 |
Picture
Picture
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