Flow cytometric analysis of BALB/c mouse bone marrow labelling Mouse c-Kit antibody at 1/500 (1 μg) dilution/ (right panel) compared with a Rat IgG2b, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD34 - FITC antibody separately.
Invivo anti-mouse c-Kit (CD117) Recombinant mAb
Invivo anti-mouse c-Kit (CD117) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | c-Kit (CD117) |
| Synonyms | Mast/stem cell growth factor receptor Kit; SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; CD117; Sl; Kit |
| Location | Cytoplasm, Cell membrane |
| Accession | P05532 |
| Clone Number | ACK2 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2b,k |
| Isotype Control | Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Application | FCM, in vivo mast cell depletion, in vivo c-Kit+ cell depletion, in vitro c-Kit neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
c-Kit (CD117) is a receptor tyrosine kinase encoded by the c-kit gene. It plays a crucial role in the development and function of various cell types including hematopoietic cells, melanocytes, and gastrointestinal interstitial cells. The ligand for c-Kit is stem cell factor (SCF), and the binding of SCF to c-Kit activates intracellular signaling pathways that promote cell proliferation, survival, differentiation, and migration. Mutations or overexpression of c-Kit are associated with several diseases such as gastrointestinal stromal tumors (GISTs) and certain types of leukemia, making it an important target for therapeutic interventions.
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FC
Flow cytometric analysis of BALB/c mouse bone marrow labelling Mouse c-Kit antibody at 1/500 (1 μg) dilution/ (right panel) compared with a Rat IgG2b, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with Lineage Cocktail - Brilliant Violet 421™ antibody separately.
