Standard data
Example of Rat Ig standard data in Assay Diluent A1.
Rat Ig isotype OneStep ELISA Kit
Rat Ig isotype OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | Ig |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Rt |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: NA; Inter-assay: NA |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (qualitatively) |
| Assay time | 60 minutes |
| Species reactivity | Rt |
Background
Immunoglobulins are crucial components in the humoral immune response of vertebrates, defending against pathogen invasion and infection. The polypeptide chains of immunoglobulins consist of two identical heavy (H) chains and two identical light (L) chains linked by inter-chain disulfide bonds. The amino-terminal region has a highly variable amino acid composition that is involved in antigen binding, while the C-terminal constant regions participate in complement binding, placental and intestinal transport, and binding to cell membranes. Based on differences in the heavy chain constant regions, six immunoglobulin heavy chain subclasses have been identified in rats: IgA, IgM, IgG1, IgG2a, IgG2b, and IgG2c.
Picture
Picture
ELISA
Standard data
Expected Result with Immunoglobulins of the Indicated Isotypes.
Protocol Diagram
