Standard curve
Example of Human IgM standard curve in Assay Diluent #1.
Human IgM OneStep ELISA Kit
Human IgM OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgM |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.3%； Inter-assay: 7.5% |
| Sample type | Cell culture supernatant, Serum, Plasm |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.074 ng/ml |
| Range | 0.39 ng/mL – 25 ng/mL |
| Recovery | Cell culture supernatant 87% Serum 103% Plasm 88% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Immunoglobulin M (IgM) is the largest of several isotypes of antibodies (also known as immunoglobulin) that are produced by vertebrates. IgM is the first antibody to appear in the response to initial exposure to an antigen; causing it to also be called an acute phase antibody. In humans and other mammals that have been studied, plasma blasts in the spleen are the main source of specific IgM production. IgM is the first immunoglobulin expressed in the human fetus (around 20 weeks) and phylogenetically the earliest antibody to develop. IgM antibodies appear early in the course of an infection and usually reappear, to a lesser extent, after further exposure. IgM antibodies do not pass across the human placenta (only isotype IgG). Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.
Picture
Picture
ELISA
Linearity
The concentrations of IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 1.6×10^4 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 418.96 ug/mL in human serum.
Linearity
#2 sample is undiluted samples are as follows: Human plasma was 1.6×10^4 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 194.45 ug/mL in human plasma.
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