Standard curve
Example of Human IgG (Total) standard curve in Assay Diluent #1.
Human IgG (Total) OneStep ELISA Kit
Human IgG (Total) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgG (Total) |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 7.3%； Inter-assay: 7.5% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 80 pg/ml |
| Range | 234 pg/ml - 15000 pg/ml |
| Recovery | Cell culture supernatant 97% Serum 88% Plasma 93% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
Immunoglobulin G (IgG) is a type of antibody. IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This kit is for the quantitative measurement of human total IgG in Human serum, plasma and supernatant from cell cultures.
Picture
Picture
ELISA
Demonstration of the tested species specificity
Linearity
The concentrations of total IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 5×10^5 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10mg/mL in human serum.
#2 sample is undiluted samples are as follows: Human plasma was 5×10^5 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.2 mg/mL in human plasma.
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Protocol Diagram
