Human CCL3/MIP-1α OneStep ELISA Kit

Catalog Number: S0C3219 Reactivity: Hu Conjugation: Brand: Starter

Price:

$420 USD

Size: 1x48T 1x96T 1x24T 5x96T 10x96T

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Antigen	CCL3/MIP-1α
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 3.6%； Inter-assay: 3.3%
Sample type	Serum; Heparin Plasma, Citrate Plasma; Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	7.31 pg/mL
Range	14.1 pg/mL – 900 pg/mL
Recovery	Serum: 112% Citrate plasma: 112% Heparin Plasma: 106% Cell Culture Supernatant: 106%
Assay time	60 minutes
Species reactivity	Hu

Background

Human CCL3 (C-C motif chemokine ligand 3), also known as macrophage inflammatory protein-1 alpha (MIP-1α), is a 92-amino acid chemotactic cytokine belonging to the CC subfamily of chemokines. It is produced by a variety of hematopoietic and non-hematopoietic cells, particularly macrophages, lymphocytes, dendritic cells, neutrophils, basophils, mast cells, and fibroblasts. As a prototypic CC chemokine, CCL3 exerts its biological functions primarily by binding to its receptors CCR1 and CCR5, mediating the chemotactic movement and activation of immune cells. In addition to its well-established proinflammatory activities in recruiting inflammatory cells such as macrophages, lymphocytes, and eosinophils, CCL3 also plays roles in wound healing, negatively regulates the proliferation of hematopoietic stem/progenitor cells (leading to its alternative designation as a stem cell inhibitor), and induces osteoclastogenesis. Its secretion increases significantly in response to inflammatory stimuli and pathological conditions. Thus, it plays a valuable role in evaluating disease pathogenesis and therapeutic interventions across a spectrum of human inflammatory and malignant conditions.

Picture

ELISA

Standard Curve
Example of Mouse NT-Pro BNP standard curve in Assay Diluent HS4. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Mouse NT-Pro BNP was evaluated in activated samples spiked with concentrations spanning the entire assay range.

Dilution linearity
. The concentration of human CCL3/MIP-1 α was measured and interpolated using the target standard curve, and the influence of sample dilution was corrected.
The leftmost bar in the figure represents the biological sample diluted to a 1.25% concentration using the detection diluent. "Un-diluted" is defined as a 1.25% biological sample. The subsequent bars represent the two-fold serial dilutions of this reference substance. In the culture supernatant of human peripheral blood mononuclear cells stimulated with 10 ug/mL PHA for 5 days, the average target concentration determined was 79.52 ng/mL.

Dilution linearity
The concentration of human IFN-γ was measured and interpolated using the target standard curve, and the influence of sample dilution was corrected.
The leftmost bar in the figure represents the biological sample diluted to a 5% concentration using the detection diluent. "Un-diluted" is defined as a 5% biological sample. After culturing human peripheral blood mononuclear cells without stimulation with 10 ug/mL PHA for 5 days, the average target concentration measured in the culture supernatant was 1.61 ng/mL.

Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Mouse NT-Pro BNP in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

Cross-reactivity
The cross-reactivity between Mouse BNP and Human BNP was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Human NT-pro BNP was tested in the Mouse NT-Pro BNP assay, as the signals remained within the background range of the assay.

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.