Standard curve
Example of E. coli Host Cell Proteins standard curve in Assay Diluent #8.
E. coli Host Cell Proteins OneStep ELISA Kit
E. coli Host Cell Proteins OneStep ELISA Kit
Price:
Regular price
$1,200 USD
Regular price
Sale price
$1,200 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | Host Cell Proteins |
| Synonyms | E. coli HCP |
| Immunogen | Recombinant Protein |
| Antibody Type | pAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.2%； Inter-assay: 5.7% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.183 ng/ml |
| Range | 0.39 ng/mL – 25 ng/mL |
| Recovery | Cell culture supernatant: 91% |
| Assay time | 60 minutes |
| Species reactivity | E. coli |
Background
E. coli host cell proteins constitute a diverse and functionally intricate group of macromolecules within the Escherichia coli cell. The host proteins of E. coli play crucial roles in various cellular processes. These proteins are involved in fundamental metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and amino acid biosynthesis, which are essential for the survival and growth of the bacterium.
Picture
Picture
ELISA
Linearity
The concentrations of E. coli Host Cell Proteins were measured and interpolated from the target standard curves and corrected for sample dilution.
Undiluted samples are as follows: E. coli expression purification sample (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 19.18 ug/mg in E. coli expression purification sample.
Linearity
Undiluted samples are as follows: unpurified recombinant E. coli protein expressed in Luria-Bertani (LB) medium (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10.23 ng/mL in cell culture medium.
Specificity
Test the purified recombinant proteins from different hosts as follows: Yeast sample(1ug/mL); E. coli sample(1ug/mL); HEK 293 sample(1ug/mL).
Specificity
The concentrations of E coli host cell proteins were quantified prior to and following the HCP removal process. The sample is one of E. coli expression purification sample from Starter catalog protein (1ug/mL).
Specificity
Test the HCP standard protein as follows: E. coli HCP standard (25ng/mL); HEK293 HCP standard (200ng/mL); CHO HCP standard (200ng/mL).
Leading Competitor comparison
Protocol Diagram
