The Key Role of CD140a Antibody in Cell Biology Research

1. Concept of CD140a Antibody and Its Molecular Basis

CD140a antibody is a highly specific immunoglobulin that binds to CD140a (platelet-derived growth factor receptor alpha, PDGFRα)—a type III tyrosine kinase receptor with an extracellular domain (five immunoglobulin-like ligand-binding regions), transmembrane domain, and intracellular tyrosine kinase domain. Produced via hybridoma or recombinant DNA technology, it specifically recognizes CD140a epitopes. Classified into monoclonal (high uniformity, specificity) and polyclonal (multiple epitope recognition, high sensitivity) antibodies, its quality (specificity, affinity, titer) directly impacts experimental reliability.

2. Research Frontiers

CD140a antibody applications span cell biology, stem cell research, signal transduction, and disease modeling. Key frontiers include refining mesenchymal stem cell (MSC) sorting using CD140a combined with other markers (CD90, CD105) for high-purity isolation, and studying PDGFRα-mediated signaling in tumor stroma and fibrosis. Antibody engineering (nanobodies, bispecific antibodies) enhances detection sensitivity and reduces non-specific binding. Translational research explores CD140a as a diagnostic biomarker for diseases like fibrosis and cancer, with multi-omics integration deepening its biological function understanding.

3. Research Significance

CD140a antibody is indispensable for advancing cell biology and translational research. It enables precise identification and sorting of MSCs, supporting tissue engineering and regenerative medicine. In signal pathway studies, it facilitates analysis of PDGFRα-mediated signaling, uncovering mechanisms of cell proliferation and differentiation. For disease research, it aids in constructing accurate tumor microenvironment and fibrosis models, identifying therapeutic targets. Its potential as a clinical biomarker bridges basic science and clinical diagnosis, addressing unmet needs in disease detection and treatment monitoring.

4. Related Mechanisms, Research Methods, and Product Applications

4.1 Cell Identification and Sorting

CD140a antibody is a gold standard for MSC isolation:

• Sorting Techniques: Immunomagnetic sorting or flow cytometry isolates CD140a-positive MSCs from heterogeneous populations, with optimized antibody concentration, incubation time, and temperature ensuring >85% osteogenic/chondrogenic/adipogenic differentiation efficiency.
• Combined Markers: Used with CD90/CD105 to refine cell subpopulations, enhancing purity for tissue regeneration research.

4.2 Signal Pathway Research

It deciphers PDGFRα-mediated signaling:

• Complex Analysis: Co-immunoprecipitation captures CD140a and interacting molecules, analyzing signaling complex composition.
• Activation Tracking: Western blot detects ~170 kDa CD140a protein; phosphorylation-specific CD140a antibodies quantify receptor activation kinetics (peak phosphorylation 5 minutes post-ligand stimulation).

4.3 Disease Model Construction

CD140a antibody supports disease research:

• Tumor Models: Labels and isolates cancer-associated fibroblasts (CAFs) in tumor stroma, establishing accurate tumor microenvironment models and tracing stromal cell origin/evolution.
• Fibrosis Models: Quantifies PDGFRα-positive myofibroblasts in fibrotic lesions (pulmonary/liver fibrosis), correlating cell numbers with disease severity.

4.4 Future Development Directions

• Antibody Engineering: Nanobodies and bispecific antibodies improve detection sensitivity and reduce non-specific binding.
• Clinical Translation: Validation as a diagnostic biomarker for fibrosis, cancer, and other diseases.
• Multi-Omics Integration: Combining with proteomics/transcriptomics to deepen PDGFRα function understanding.

4.5 Product Applications in Advanced Research

ANT BIO PTE. LTD.’s CD140a antibodies support key scenarios:

• Stem Cell Research: MSC isolation, differentiation, and developmental biology studies.
• Signal Transduction: PDGFRα pathway activation, complex formation, and kinase activity analysis.
• Disease Research: Tumor stromal interaction, fibrosis mechanism exploration, and preclinical model construction.

5. Brand Mission

ANT BIO PTE. LTD. is dedicated to empowering global cell biology, stem cell, and translational research through innovative, high-quality reagents. We strive to develop cutting-edge antibodies, proteins, kits, and tools that enable researchers to unravel cellular mechanisms, construct disease models, and advance therapeutic development. Our mission is to accelerate scientific discovery, facilitate the translation of basic research to clinical applications, and improve human health by providing reliable, reproducible, and high-performance research solutions. With a commitment to excellence, innovation, and customer-centricity, we aim to be a trusted partner for researchers advancing the frontiers of precision medicine.

6. Related Product List

Core Advantages of ANT BIO PTE. LTD.’s CD140a Antibodies

• High Specificity and Functional Recognition: Specifically binds mouse/human CD140a (PDGFRα), validated for MSCs, fibroblasts, and glial cells—enabling precise cell identification.
• Exceptional Stability and Consistency: Strict quality control ensures minimal batch-to-batch variation and reliable performance across flow cytometry, IHC, and WB.

7. AI Disclaimer

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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.