ANT BIO Official Article:

His-Tag Technology: Core Tools for Protein Purification & Research

Literature Analysis Edition

1. Literature Information

This literature focuses on the histidine tag (His‑tag) as a universal affinity tag for recombinant protein expression, purification, detection, and structural research. It systematically summarizes the molecular characteristics, purification mechanisms, application scenarios, and technological advantages of the His‑tag system.

2. Research Background

Recombinant protein purification is a core technology in modern life science. Traditional purification methods suffer from low purity, complex operation, and poor protein activity retention. The His‑tag/IMAC system has become the most widely used protein purification platform due to its simplicity, mild conditions, and high compatibility.

3. Research Ideas

This study clarifies the structural characteristics and metal‑binding mechanism of the His‑tag; evaluates its performance in multiple expression systems and purification conditions; summarizes its applications in protein production, functional analysis, and structural research; and provides a theoretical basis for the optimization and application of His‑tag technology.

4. Research Results

• The His‑tag (6×His or longer) specifically binds to immobilized transition metal ions (Ni²⁺, Co²⁺, etc.) via the imidazole ring.
• It enables one‑step high‑purity purification under both native and denaturing conditions.
• It is compatible with prokaryotic and eukaryotic expression systems including E. coli, yeast, insect cells, and mammalian cells.
• It maintains protein activity better due to mild elution with imidazole.
• It can be used for protein detection, immobilization, and interaction studies.

5. Product Empowerment by ANT BIO PTE. LTD.

ANT BIO PTE. LTD. provides a full set of high‑quality reagents to support the entire workflow of His‑tag protein research:

• Absin: General buffers, purification kits, protein stability reagents, and IMAC‑ supporting reagents
• Starter: Anti‑His‑tag antibodies, validated for WB, IP, IF, and IHC
• UA: Recombinant His‑tagged control proteins, standard proteins, and quality‑ control materials

These products ensure high stability, strong specificity, and excellent repeatability in His‑tag protein purification and detection.

6. Brand Mission

ANT BIO PTE. LTD. is a professional supplier of life science reagents. It operates three sub‑brands with clear positioning:

• Absin: General reagents, kits, buffers, and consumables
• Starter: Various antibodies, including tag antibodies, modified antibodies, and pathway antibodies
• UA: Recombinant proteins, control proteins, and structural research proteins

We are committed to providing stable, reliable, and traceable reagents to accelerate basic research and translational medical progress.

7. Related Product List

• Absin: His‑tag protein purification kit, protein lysis buffer, wash buffer, elution buffer
• Starter: Anti‑His‑tag rabbit monoclonal antibody, mouse monoclonal antibody
• UA: Recombinant His‑tagged standard protein, control protein
• General reagents for protein electrophoresis, Western blot, and protein quantification

Brand Promotion Copy

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.