Empowering Universal Blood Cancer Therapies and Immunology Research: The Core Role of Mouse CD45 Antibodies by ANT BIO PTE. LTD.

1. Concept

CD45, formally known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC) or Leukocyte Common Antigen (LCA), is a transmembrane glycoprotein with a molecular weight of 180–220 kDa. It is expressed on all hematopoietic lineage cells except mature erythrocytes, including T cells, B cells, natural killer cells, monocytes, macrophages, granulocytes, and their precursors. As a key molecular switch in immune cell signaling, CD45 exerts intracellular protein tyrosine phosphatase activity to dephosphorylate and activate Src family kinases (e.g., Lck, Fyn in T cells; Lyn in B cells), positively regulating T cell receptor (TCR) and B cell receptor (BCR) signaling pathways. It also modulates cytokine receptor signaling and lymphocyte survival, with dysfunction linked to immune deficiencies, autoimmune diseases, and hematologic malignancies. Mouse CD45 antibodies have emerged as indispensable tools for immunology research and the development of universal blood cancer therapies.

2. Research Frontiers

A leading research frontier focuses on overcoming the "fratricide" challenge in CD45-targeted CAR-T therapy. Innovative strategies, such as CRISPR-mediated base editing, modify CD45 epitopes on CAR-T cells and hematopoietic stem cells (HSCs) to retain their physiological functions while evading CAR recognition. This enables "stealth" CAR-T cells to eliminate malignant hematopoietic cells without attacking normal immune cells or HSCs, paving the way for universal hematologic malignancy treatments.

Another active area of investigation involves leveraging CD45 isoforms for precise immune cell subtyping. Alternative splicing of the CD45 gene produces isoforms (e.g., CD45RA, CD45RB, CD45RO) with differential expression across immune cell subsets and activation states. Researchers are developing isoform-specific antibodies to refine immunophenotyping, supporting studies on immune cell development, differentiation, and dysfunction in disease.

Preclinical research frontiers also include exploring CD45 as a target for antibody-drug conjugates (ADCs) and bispecific antibodies, expanding its utility beyond CAR-T therapy for blood cancer treatment.

3. Research Significance

Investigating CD45 and utilizing mouse CD45 antibodies holds profound significance for both immunology research and translational medicine. In basic research, these antibodies enable the identification, sorting, and subtyping of immune cells, advancing our understanding of immune system function, development, and dysregulation. This knowledge forms the foundation for addressing immune-related diseases.

In translational medicine, CD45-targeted therapies have the potential to revolutionize blood cancer treatment by offering universal, off-the-shelf options. Mouse CD45 antibodies play a critical preclinical role, validating target expression, evaluating CAR-T cell efficacy and safety, and monitoring immune reconstitution post-treatment. Their use accelerates the translation of innovative therapies to clinical practice, benefiting patients with relapsed/refractory hematologic malignancies.

4. Related Mechanisms, Research Methods, and Product Applications

Related Mechanisms

CD45’s core function lies in regulating immune cell signaling. As illustrated in the diagram, CD45 dephosphorylates Src family kinases, activating TCR/BCR signaling cascades that drive lymphocyte activation, proliferation, and effector function. Its isoforms (generated via alternative splicing) exhibit tissue- and activation-specific expression—for example, naïve T cells express CD45RA, while memory T cells express CD45RO—enabling precise immune cell subtyping. In CD45-targeted CAR-T therapy, unmodified CAR-T cells recognize CD45 on both malignant and normal hematopoietic cells, leading to fratricide and myelosuppression. Epitope editing circumvents this by altering CD45’s antigenic structure on normal cells.

Research Methods and Product Applications

Mouse CD45 antibodies are versatile tools supporting diverse research and preclinical applications. ANT BIO PTE. LTD.’s Alexa Fluor® 647 Mouse Anti-Rat CD45 Antibody (Catalog No.: S0B8252) stands out for its high specificity, superior fluorescence performance, and multi-platform compatibility, making it an essential reagent for immunology research and drug development.

Key Applications of the Antibody:

• Immune Cell Identification and Sorting: As a pan-leukocyte marker, the antibody enables efficient isolation and enrichment of leukocytes from complex tissues (e.g., spleen, lymph nodes, tumors) via flow cytometry or immunomagnetic sorting, supporting downstream functional and transcriptional analyses.
• Precise Immune Cell Subtyping: Combined with isoform-specific or subset markers (e.g., CD3, CD4, CD19), the antibody refines subtyping of T cells, B cells, and myeloid cells, aiding studies on immune cell differentiation and activation.
• Disease Model Analysis: In mouse models of autoimmune diseases, infections, and hematologic malignancies, the antibody monitors immune cell infiltration, proportion, and function in target organs, evaluating disease progression and treatment responses.
• Preclinical Therapy Evaluation: In CD45-targeted CAR-T or ADC development, the antibody validates target expression, assesses malignant cell clearance, and monitors immune reconstitution, serving as a core tool for efficacy and safety assessment.

Core Advantages of the Antibody:

• Superior Fluorescence Performance: The Alexa Fluor® 647 label emits at ~668 nm, offering high brightness, photostability, and minimal spectral overlap with common fluorochromes (e.g., FITC, PE). This ensures clear leukocyte gating in complex multicolor flow cytometry panels.
• Exceptional Specificity: The antibody specifically recognizes CD45 on all leukocytes, with no cross-reactivity to erythrocytes or platelets. It enables accurate live cell gating, excluding debris and non-leukocytes for reliable immunophenotyping.
• Ready-to-Use Convenience and Batch Consistency: Pre-titrated and provided in a ready-to-use buffer, the antibody simplifies experiments. Rigorous quality control ensures consistent fluorescence intensity and specificity across batches, guaranteeing reproducible results for long-term studies.

CD45 Isoform Expression and Applications:

5. Brand Mission

ANT BIO PTE. LTD. is dedicated to advancing life science research and clinical diagnostics by providing high-performance, high-value core reagents and comprehensive solutions. Leveraging advanced development platforms—including recombinant rabbit monoclonal antibody, recombinant mouse monoclonal antibody, rapid mouse monoclonal antibody, and recombinant protein development platforms (E.coli, CHO, HEK293, Insect Cells), as well as the One-Step ELISA Platform and PTM Pan-Modification Antibody Platform—the company adheres to stringent quality standards and has successfully obtained EU 98/79/EC certification, ISO9001 certification, and ISO13485 certification. ANT BIO PTE. LTD. strives to support researchers and clinicians worldwide in their pursuit of scientific breakthroughs, improved patient care, and the development of innovative therapies for hematologic malignancies and immune-related diseases.

6. Related Product List

For more information on our extensive product portfolio, including conventional antibodies, pairing antibodies, CDx antibodies, ELISA kits, proteins, and custom antibody services, please visit our official website.

7. AI Disclaimer

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