Can the p90RSK recombinant rabbit monoclonal antibody elucidate the multidimensional regulation of kinase signaling?

Concept

p90 Ribosomal S6 Kinase (p90RSK), a key serine/threonine kinase of the AGC superfamily, acts as a pivotal downstream effector of the Ras-MAPK signaling pathway and a core nodal molecule in the multidimensional regulation of cellular kinase signaling cascades. Characterized by two functionally independent kinase domains—an N-terminal domain responsible for substrate phosphorylation and a C-terminal domain governing self-activation—p90RSK modulates a broad spectrum of core cellular processes including proliferation, survival, differentiation and metabolism via phosphorylating diverse substrates (transcription factors, ribosomal proteins, cell cycle regulators and epigenetic modifiers).

Aberrant activation of the p90RSK signaling pathway is closely implicated in the initiation, progression and drug resistance of various human cancers, as well as the pathogenesis of neurological disorders. The p90RSK Recombinant Rabbit Monoclonal Antibody is an indispensable research tool, enabling precise detection of p90RSK in different activation states, splice variants and subcellular localizations. This high-performance antibody provides critical support for elucidating the spatiotemporal dynamic functions and regulatory mechanisms of p90RSK in complex kinase signaling networks, and serves as a core reagent for disease mechanism research and anti-tumor drug development.

Research Frontier

Current research on p90RSK and its role in kinase signaling regulation is advancing rapidly at the intersection of molecular cell biology, oncology, neurobiology and translational medicine, with key cutting-edge directions shaping the field:

1. p90RSK as a therapeutic target for cancer and neurological diseases: Research is focused on clarifying the isoform-specific and context-dependent oncogenic mechanisms of p90RSK (RSK1-4), as well as its role in synaptic plasticity and neuronal survival. This work drives the development of selective p90RSK inhibitors for overcoming cancer drug resistance and treating neurodegenerative disorders.
2. Precision antibody engineering for p90RSK detection: A major research focus is the development of antibodies that distinguish total p90RSK protein from its phosphorylated active forms (Thr359, Ser380), as well as isoform-specific antibodies for RSK1-4. Additionally, antibodies capable of recognizing dynamic conformational states of p90RSK are in high demand for studying its activation and subcellular shuttling mechanisms.
3. Multidimensional regulation of p90RSK in kinase signaling networks: Research is exploring the complex crosstalk between p90RSK and other signaling pathways (PI3K/Akt, mTOR/p70S6K), as well as the negative feedback regulation mechanisms that fine-tune p90RSK activity. This work aims to unravel the multidimensional regulatory role of p90RSK in cellular signaling homeostasis.
4. Spatiotemporal dynamics of p90RSK subcellular localization: A key frontier is defining the molecular mechanisms underlying p90RSK nucleocytoplasmic shuttling and the functional consequences of its subcellular localization—including cytoplasmic regulation of cell proliferation and nuclear phosphorylation of transcription factors such as CREB and c-Fos.
5. Application of p90RSK antibodies in high-throughput drug screening: The integration of p90RSK antibodies into phosphorylation-specific ELISA assays and high-content imaging systems is accelerating the development and preclinical evaluation of p90RSK-targeted drugs, with a focus on improving target engagement assessment and efficacy validation.

Research Significance

Elucidating the multidimensional regulatory role of p90RSK in kinase signaling networks and developing high-quality p90RSK Recombinant Rabbit Monoclonal Antibodies hold profound scientific and translational significance across multiple research fields:

• Mechanistic research of kinase signaling cascades: p90RSK is a critical effector of the Ras-MAPK pathway, and specific antibodies enable precise detection of its activation state, subcellular localization and protein-protein interactions. This provides critical insights into the multidimensional regulation of cellular signaling networks and the crosstalk between major signaling pathways.
• Oncology research and cancer therapy development: Abnormal p90RSK activation is a hallmark of many human cancers and is closely linked to drug resistance. p90RSK antibodies facilitate the characterization of this kinase’s role in tumorigenesis and drug resistance, and support the validation of p90RSK as a prognostic biomarker and therapeutic target for cancer patients.
• Neurological disease research: p90RSK is a key regulator of synaptic plasticity and neuronal survival, and p90RSK antibodies enable the analysis of its expression and activation in different brain regions and neuronal subpopulations. This work advances the understanding of the molecular mechanisms underlying neurodegenerative diseases and provides new therapeutic targets.
• Preclinical and clinical drug development: p90RSK antibodies are essential tools for evaluating the efficacy of p90RSK-targeted inhibitors at all stages of drug development—from in vitro cell-based assays to in vivo animal models and clinical tissue analysis. They enable quantitative assessment of target engagement and the identification of drug resistance mechanisms, accelerating the translation of novel therapeutics to the clinic.
• Advancement of biological detection methodologies: The development of highly specific and sensitive p90RSK Recombinant Rabbit Monoclonal Antibodies sets a benchmark for antibody engineering targeting multi-domain, conformationally dynamic kinases. It advances detection methodologies for Western Blot, immunofluorescence, immunohistochemistry and flow cytometry across the life sciences.

Related Mechanisms and Product Applications

Core Mechanisms of p90RSK in Kinase Signaling Regulation

p90RSK functions as a critical downstream effector of the Ras-MAPK signaling pathway, with its activation dependent on a sequential series of phosphorylation events mediated by ERK1/2 at key residues (Thr359, Ser380) and intramolecular interactions between its two kinase domains. The C-terminal kinase domain is first phosphorylated by ERK, triggering its activation and subsequent phosphorylation of the N-terminal kinase domain, which then mediates the phosphorylation of downstream substrate proteins.

As a multifunctional kinase, p90RSK phosphorylates a diverse array of substrates, linking the Ras-MAPK pathway to the regulation of transcription, translation, cell cycle progression and metabolism. In the cytoplasm, p90RSK regulates cell proliferation and survival by phosphorylating ribosomal proteins and cell cycle regulators; upon activation, a subset of p90RSK translocates to the nucleus, where it phosphorylates transcription factors (CREB, c-Fos) to modulate gene expression. The spatiotemporal dynamics of p90RSK activation and subcellular localization are critical for its multidimensional regulatory role in kinase signaling networks, and are tightly regulated by upstream signals and negative feedback mechanisms.

Under pathological conditions, constitutive activation of p90RSK disrupts normal signaling homeostasis, driving tumor cell proliferation, invasion and drug resistance in cancer, and impairing synaptic plasticity and neuronal survival in neurological disorders. This makes p90RSK a critical therapeutic target for the development of novel anti-tumor and neuroprotective drugs.

Key Structural Features for p90RSK Antibody Design

The molecular complexity and dynamic nature of p90RSK impose stringent requirements for the design of high-performance Recombinant Rabbit Monoclonal Antibodies, with key structural and functional features to consider:

1. Dual kinase domain recognition: p90RSK’s two functionally independent kinase domains (N-terminal and C-terminal) require antibody design that can target either conserved regions for total protein detection or activation-specific phosphorylation sites (Thr359, Ser380) for functional activity analysis.
2. Discrimination of activation states: Antibodies must distinguish between the inactive and phosphorylated active forms of p90RSK, with phosphorylation-specific antibodies enabling the detection of kinase activity and spatiotemporal activation dynamics.
3. Splice variant and isoform coverage: p90RSK has four major isoforms (RSK1-4) and multiple splice variants; antibody design should consider either conserved regions for pan-p90RSK detection or unique regions for isoform-specific recognition, based on research needs.
4. Conformational epitope recognition: p90RSK exhibits dynamic conformational changes during activation and subcellular shuttling; leveraging the rabbit immune system’s unique ability to generate antibodies against conformational epitopes is critical for detecting the native, functional form of the kinase.
5. Post-translational modification compatibility: p90RSK undergoes multiple post-translational modifications beyond phosphorylation; immunogen design should cover different modification states to enrich diverse antibody clones and ensure comprehensive detection.

Broad Applications of ANT BIO PTE. LTD.’s p90RSK Recombinant Rabbit Monoclonal Antibody

ANT BIO PTE. LTD.’s p90RSK Recombinant Rabbit Monoclonal Antibody (Starter sub-brand, Product No.: S0B0782) is engineered with high specificity, high affinity and excellent stability, targeting the conserved kinase domain of p90RSK and enabling specific recognition of total p90RSK protein (RSK1-4 subtypes) across multiple species (human, mouse, rat). Validated for use across a diverse range of experimental platforms, this antibody is a core research tool for elucidating the multidimensional regulation of p90RSK in kinase signaling networks, with its key applications including:

1. Kinase Signaling Pathway Mechanism Research

As a critical effector of the Ras-MAPK pathway, p90RSK’s activation state reflects upstream signal intensity and duration, and this antibody enables comprehensive analysis of p90RSK function in signaling networks:

• It supports quantitative Western Blot analysis of total p90RSK and phosphorylated active forms, allowing the inference of ERK activation patterns and negative feedback regulation mechanisms by comparing phosphorylation dynamics under different stimulatory conditions.
• It enables immunofluorescence imaging of p90RSK subcellular localization, revealing the nucleocytoplasmic shuttling dynamics of the kinase—including cytoplasmic retention in resting states and nuclear translocation upon activation—and visualizing the subcellular distribution of activated p90RSK using phosphorylation-specific detection.
• It facilitates immunoprecipitation combined with kinase activity assays, enabling the enrichment of endogenous p90RSK protein complexes and the detection of kinase activity toward downstream substrate proteins, uncovering novel p90RSK-interacting partners and substrates.

2. Disease Mechanism Research in Cancer and Neuroscience

Abnormal p90RSK activation is closely linked to cancer and neurological disorders, and this antibody is a powerful tool for elucidating the molecular mechanisms of these diseases:

• Oncology research: It supports immunohistochemical staining of tumor tissues to evaluate p90RSK expression and activation levels, and correlate these with clinicopathological parameters (e.g., tumor stage, grade, patient prognosis). In breast cancer and lung cancer research, it enables the detection of p90RSK activation in drug-resistant cell lines and the analysis of signaling network reprogramming mechanisms underlying hormone therapy and TKI resistance.
• Neuroscience research: It is used for immunostaining of brain tissue sections to reveal p90RSK expression and distribution in different brain regions and neuronal subpopulations, providing an anatomical basis for elucidating its role in synaptic plasticity and neurodegenerative diseases. It also enables the analysis of p90RSK activation dynamics in neuronal cells under physiological and pathological conditions, uncovering its role in neuronal survival and death.

3. Anti-Tumor and Neuroprotective Drug Development

As a promising therapeutic target, p90RSK requires reliable research tools to assess drug efficacy and target engagement, and this antibody serves as a core reagent for all stages of p90RSK-targeted drug development:

• It is used to construct phosphorylation-specific ELISA assays for the quantitative measurement of p90RSK activity changes following inhibitor treatment, enabling high-throughput screening of potential drug candidates.
• In cell-level efficacy evaluations, it is combined with downstream substrate phosphorylation detection to establish robust correlations between target engagement and phenotypic effects (e.g., cell proliferation, apoptosis).
• In in vivo preclinical studies, it supports immunohistochemical staining of tumor and brain tissues to evaluate the inhibition efficiency and duration of action of p90RSK inhibitors on the p90RSK signaling pathway.
• It screens synergistic combination therapy strategies by detecting the additive inhibitory effects of different drug combinations on p90RSK activation and downstream signaling networks, providing experimental evidence for optimizing treatment regimens.
• In drug resistance research, it tracks the compensatory activation of the p90RSK signaling pathway in resistant cells, guiding the design of novel intervention strategies to overcome resistance.

4. Multi-Platform Basic and Translational Research

Validated for use in Western Blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC) and flow cytometry (Flow), this antibody supports comprehensive p90RSK detection and functional analysis across diverse experimental scenarios:

• It enables sensitive and accurate detection of p90RSK expression and activation in cell lines, primary cells, FFPE tumor tissues and brain tissue sections across multiple species, with high affinity for all major p90RSK isoforms (RSK1-4).
• Its low background signal and high specificity ensure reliable results in both qualitative and quantitative assays, making it a core tool for Ras-MAPK signaling pathway research, cell proliferation and differentiation studies, translation regulation research, and tumor biology research.
• Its excellent stability and batch-to-batch consistency provide reliable raw materials for long-term research projects, multi-center collaborative experiments and experimental methodological development, meeting the rigorous demands of both basic scientific research and clinical translational research.

Core Product Advantages

Professional Technical Support

ANT BIO PTE. LTD. provides comprehensive and expert technical support for the p90RSK Recombinant Rabbit Monoclonal Antibody, including detailed product technical documentation covering:

• Isoform recognition validation data (cross-reactivity analysis for RSK1-4 subtypes)
• Species cross-reactivity validation data (human, mouse, rat, etc.)
• Multi-platform application protocols (optimized recommended conditions for WB/IP/IF/IHC/Flow)
• Typical application examples (tumor tissue IHC staining, Ras-MAPK signaling pathway research data, neuronal tissue immunofluorescence imaging)

Our professional technical team offers efficient and personalized support for experimental design, kinase signaling pathway research, tumor biomarker analysis, neurological disease mechanism research and drug development studies, ensuring the success of your research projects.

ANT BIO PTE. LTD. has established a mature and advanced rabbit immunization and single B-cell antibody development platform, covering the entire R&D workflow from rational antigen design and immunization strategy optimization to single B-cell high-throughput sorting, antibody gene cloning, recombinant expression, and multi-application validation. We provide systematic antibody customization solutions for various research application scenarios, tailored to meet the specific research needs of the global scientific community in cell biology, oncology, neuroscience and drug development.

Brand Mission

At ANT BIO PTE. LTD., our core mission is to empower life science breakthroughs by developing and providing high-quality, innovative and reliable biological reagents and comprehensive research solutions for scientists and researchers worldwide. Leveraging our advanced recombinant antibody development platforms—including a state-of-the-art rabbit immunization and single B-cell sorting system—and rigorous quality control protocols, we engineer cutting-edge research tools for key signaling molecules such as p90RSK, addressing the critical research needs of the scientific community in kinase signaling research, oncology, neuroscience and translational drug development.

Our three specialized sub-brands form a comprehensive and integrated product ecosystem that covers the full spectrum of life science research needs, supporting every stage of the experimental workflow from basic molecular mechanistic research to preclinical translational development:

• Absin: Specializes in high-quality general life science reagents and research kits, including molecular biology, cell biology and immunology reagents, providing essential experimental tools for sample preparation, signal detection and functional validation in p90RSK and kinase signaling pathway research.
• Starter: Our flagship antibody sub-brand, focused on the development of premium polyclonal, monoclonal and recombinant antibodies—including the p90RSK Recombinant Rabbit Monoclonal Antibody. Starter antibodies are engineered for high specificity, high affinity and multi-platform versatility, rigorously validated for various research applications and designed to meet the rigorous demands of basic scientific research and clinical translational research.
• UA: Dedicated to the development and production of high-purity recombinant proteins, including cytokines, growth factors and key signaling molecules (kinases, receptors), providing research-grade reagents for cell culture, pathway activation and in vitro functional assays in p90RSK, Ras-MAPK and cancer biology research.

We are committed to being a trusted and reliable research partner for scientists worldwide, providing not only superior quality biological reagents but also expert technical support and customized solution design. By prioritizing innovation, quality and customer-centricity, we accelerate the pace of scientific discovery and the development of novel therapeutic strategies for human diseases, bridging the critical gap between basic scientific research and clinical translation.

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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.