{"product_id":"unione-tr-fret-human-tnf-tnfr2-binding-kit-ua086008","title":"UniOne® TR-FRET Human TNF-α\/TNFR2 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eThis kit employsHomogeneous Time-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween TNF-α and TNFR2. The method enables simple and rapid high-throughputscreening of inhibitors and antibody blockers.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size:11.0pt;color:black\"\u003eAs shown in thefigure, the TNF-α\/TNFR2 interaction is detected using an Eu-labeled anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled TNF-α protein (TR-FRETacceptor). Binding of TNF-α to TNFR2 brings the donor antibody into proximitywith the acceptor protein. Excitation of the donor antibody triggersFluorescence Resonance Energy Transfer (FRET) to the acceptor, resulting inspecific emission at 665 nm. This specific signal is proportional to the levelof TNF-α\/TNFR2 interaction. This homogeneous assay is simple to perform andrequires no wash steps.\u003c\/span\u003e\u003cspan style=\"font-size:11.0pt\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/product.template\/20289050\/d56942e8-3b74-4090-a93e-d1e49944b314.png?access_token=363abac1-8e97-4f7b-b34e-5d6afc6d8a1e\" class=\"img img-fluid o_we_custom_image\" style=\"width: 50%;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth style=\"border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2500 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e10000 TESTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003cth style=\"border-left:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTNF\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eΑ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1-TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e200 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e800 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu-dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e250 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1000 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eμ\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40 mL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:7.5pt 7.5pt 7.5pt 7.5pt;\" valign=\"bottom\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e-80\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e°\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eC\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cspan style=\"background-color:rgb(255,255,255);color:rgb(0,0,0);\"\u003e\u003cspan style=\"-webkit-text-stroke-width:0px;display:inline !important;float:none;font-family:宋体;font-size:14.6667px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:left;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;\"\u003e* Avoid repeated freeze-thaw cycles. Aliquot after first thaw and store according to conditions indicated. Shelf life is one year.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThaw all reagents to room temperature before use (equilibrate at room temperature for at least\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e30min\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well low-volume plate reaction volume is\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e20μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (reagent volumes for the reaction system are shown in the table). Calculate the required volume for the experiment before preparation and prepare as needed; the following preparation is for reference only, using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e500\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ereactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.1 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation of Buffer (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2mL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e Detection buffer \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×)\u003c\/span\u003e\u003cspan style=\"color:windowtext;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eadd\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e18mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003esterile water, mix well and set aside;\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp class=\"Default\" style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eReagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:10.15pt;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eVolume (\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003eμL\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTest Sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDilute the test sample to the desired concentration using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e according to the reaction system.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eIf\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eis used, ensure the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econcentration is consistent across all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTNFΑ-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.5mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTag1- TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e40μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003estock solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4mL ,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;font-weight:normal;\"\u003e\u003cstrong\u003emix well and set aside.\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:114.2pt;\" width=\"152\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:232.85pt;\" width=\"310\" valign=\"top\"\u003e\n\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTake\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e50μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003edilute to\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2500μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, mix well and set aside.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:61.7pt;\" width=\"82\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp class=\"Default\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e1.2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSerial Dilution of Test Samples\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-indent:21.0pt;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ePrepare test samples using\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e. If the sample stock is in\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, it is recommended to maintain a consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003econcentration in the system,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e(\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ethe detection buffer does not contain\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e; please add\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eto the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eaccording to the dilution of the standard or test sample)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, with a maximum concentration not exceeding\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e2 \u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eSample Addition and Controls\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eAddition order for experimental wells:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003etest sample,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eworking solution,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, sequentially added into the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e384\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e-well low-volume plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.2 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMinimum control well:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e),\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.3 \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMaximum control well:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e),\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e,\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2.4 Eu only\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15μL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× buffer plus\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ediluted\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eAnti-Tag1 Eu dye antibody\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eTable\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e4. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample Addition and Controls\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" width=\"59\" valign=\"top\"\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMinimum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:115.75pt;\" width=\"154\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eMaximum Control\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:102.05pt;\" width=\"136\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-left:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:67.5pt;\" width=\"90\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eEu Only\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" rowspan=\"2\" width=\"59\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:115.75pt;\" width=\"154\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:102.05pt;\" width=\"136\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSerially diluted test sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:67.5pt;\" rowspan=\"3\" width=\"90\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e15uL 1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:19.25pt;\"\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:19.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:96.8pt;\" width=\"129\" valign=\"top\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e× \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (containing\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;height:19.25pt;padding:0cm 5.4pt 0cm 5.4pt;width:217.8pt;\" colspan=\"2\" width=\"290\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e8μL Tag1- TNFR2 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" rowspan=\"2\" width=\"59\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:314.6pt;\" colspan=\"3\" width=\"419\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL TNFΑ-01A protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:382.1pt;\" colspan=\"4\" width=\"509\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e5μL Anti-Tag1 Eu dye antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border-top:none;border:solid windowtext 1.0pt;padding:0cm 5.4pt 0cm 5.4pt;width:44.0pt;\" width=\"59\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eStep 3\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:solid windowtext 1.0pt;border-left:none;border-right:solid windowtext 1.0pt;border-top:none;padding:0cm 5.4pt 0cm 5.4pt;width:382.1pt;\" colspan=\"4\" width=\"509\" valign=\"top\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSeal the plate with a sealing film\u003cstrong\u003e,\u003c\/strong\u003e and incubate at room temperature for\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2h\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eFor negative controls and quality control, it is recommended to use\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ea solution with the same matrix as the test sample to replace\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDetection buffer\u003c\/span\u003e\u003cspan style=\"color:black;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e*\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eEnsure consistent\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eDMSO\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eor other matrix concentrations across all detection wells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e\u003cstrong\u003e3.\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eDetect using a microplate reader compatible with\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (excitation wavelength at\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e320nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, emission wavelengths at\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e and\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e \u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Result Calculation]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e1. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate signal value:\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e665nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal divided by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e620nm\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003efluorescence signal, then multiplied by\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e10000\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e, i.e.,\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eSignal Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (665\/620)*10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e2. \u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate the δ value based on the signal value:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eδ Value\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= (Std-NC)\/NC*100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e3.\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eCalculate\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;text-autospace:none;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eCV\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e (\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e%\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e)\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e= Standard Deviation\/Mean Ratio\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003e×\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003e100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"background-color:white;line-height:150%;margin:0cm 9.0pt 0cm 0cm;\"\u003e\u003cspan style=\"color:#7030A0;\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;line-height:150%;\"\u003e\u003cstrong\u003e[Data Example]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\" align=\"left\"\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003eThe following data cannot replace data obtained from actual experiments and is provided for illustration only. Results may vary depending on the\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\" lang=\"EN-US\"\u003eTR-FRET\u003c\/span\u003e\u003cspan style=\"font-family:宋体;font-size:11.0pt;\"\u003ecompatible instrument.\u003c\/span\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"500T","offer_id":42332757131339,"sku":null,"price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332757164107,"sku":null,"price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332757196875,"sku":null,"price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_fcdd2366-dd5a-4f0d-bb31-4b5fb0781228.png?v=1780633656","url":"https:\/\/www.antbioinc.com\/products\/unione-tr-fret-human-tnf-tnfr2-binding-kit-ua086008","provider":"AntBio","version":"1.0","type":"link"}