{"product_id":"unione-tr-fret-human-nek7nlrp3-binding-kit-ua086135","title":"UniOne® TR-FRET Human NEK7\/NLRP3 Binding Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eASSAY PRINCIPLE\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between\u003c\/span\u003e\u003cspan\u003e human\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNEK7\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003ehuman NLRP3\u003c\/span\u003e\u003cspan\u003e. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between \u003c\/span\u003e\u003cspan\u003eNEK7\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003eNLRP3\u003c\/span\u003e\u003cspan\u003e simply and rapidly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAs shown in the figure below, the interaction between \u003c\/span\u003e\u003cspan\u003eNEK7\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003eNLRP3\u003c\/span\u003e\u003cspan\u003e was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). Since \u003c\/span\u003e\u003cspan\u003eNEK7 binds to NLRP3\u003c\/span\u003e\u003cspan\u003e, the donor is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. \u003c\/span\u003e\u003cspan\u003eThe screened compounds block the binding of NEK7 to NLRP3, preventing FRET signal generation. The stronger the compound's ability to inhibit the interaction, the lower the signal intensity. The signal intensity is directly proportional to the extent of NEK7-NLRP3 interaction. No washing step is required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6a91d085-02c1-4fbb-8a0b-ce8737c3fa28.jpeg\" style=\"margin-left:30px; width:484px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Composition and Storage Conditions】\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse:collapse;width:auto;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003econcentration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e100T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2500T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10000T\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eStorage \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-NLRP3 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- NEK7 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag1 Eu antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e Ac antibody\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003cspan\u003e×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e25\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e125\u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e00μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003em\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e15\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e75\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e3\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e-80℃\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eNote: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【Process and Operations】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e、Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e.1 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eCompositions \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eequilibration\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eBefore use, dissolve all reagents at room temperature (allow at least 30 minutes for temperature equilibration). For the 384-well plate assay, use 20 μL of reaction mixture (the reagent quantities are listed in the table below). Calculate the required volume prior to preparation accordingly. The following preparation steps are provided for reference only, using a 500-well plate as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 1. Reagent Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComposition \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eConfiguration\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eμL\/well\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag1-NLRP3 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake \u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e0 μL of the \u003c\/span\u003e\u003cspan\u003e10\u003c\/span\u003e\u003cspan\u003e0× \u003c\/span\u003e\u003cspan\u003eTag1-NLRP3 protein\u003c\/span\u003e\u003cspan\u003e, add 1.9\u003c\/span\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e mL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTag2- NEK7 protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eTake \u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e0 μL of \u003c\/span\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e0× \u003c\/span\u003e\u003cspan\u003eTag2- NEK7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e, add 1.9\u003c\/span\u003e\u003cspan\u003e6\u003c\/span\u003e\u003cspan\u003emL of Detection buffer to dilute it to 1× concentration, and mix thoroughly for later use.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eAntibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eTake 25 μL of the 100× \u003c\/span\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e Eu antibody\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003estock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; take 25 μL of the 100× \u003c\/span\u003e\u003cspan\u003eAnti-Tag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e Ac antibody\u003c\/span\u003e\u003cspan\u003e stock solution, add 2475 μL of Detection buffer to dilute to 2.5 mL, and mix thoroughly; combine the two solutions in a 1:1 ratio to obtain the Antibody Mix.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e1.2 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eGradient dilution of the sample \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrepare test samples using 1× Detection Buffer. If the sample stock solution is prepared in DMSO, it is recommended to keep the DMSO concentration consistent across all reaction systems. Since the Detection Buffer does not contain DMSO, add DMSO to the 1× Detection Buffer according to the sample dilution ratio, with a maximum final concentration of 2%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e、Sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.1 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSam\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eple：\u003c\/span\u003e\u003cspan\u003eAdd 4 μL of \u003c\/span\u003e\u003cspan\u003eTag1-NLRP3 \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e working solution, 4 μL of \u003c\/span\u003e\u003cspan\u003eTag\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003eNEK7 \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e working solution, 2 μL of the gradient-diluted sample , and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e2\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e Blank：\u003c\/span\u003e\u003cspan\u003e4 μL of \u003c\/span\u003e\u003cspan\u003eTag1-NLRP3\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e working solution, 4 μL of \u003c\/span\u003e\u003cspan\u003eTag2-NEK7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e working solution, 2 μL of \u003c\/span\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003cspan\u003e, and 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003e2.3 \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003econtrol\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e：\u003c\/span\u003e\u003cspan\u003e10μL Detection buffer\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eand 10 μL of the mixed Antibody Mix sequentially into the 384-well plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eAfter adding all samples, centrifuge, seal with a cover membrane, and incubate at room temperature \u003c\/span\u003e\u003cspan\u003efor 2\u003c\/span\u003e\u003cspan\u003e hours.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eTable 3. \u003c\/span\u003e\u003cspan\u003eSample and control spotting process\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;background-color:#8BAAD2;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eNegative control(\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNC)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003egradient-diluted sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eDetection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"5\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e1\u003c\/span\u003e\u003cspan\u003e0\u003c\/span\u003e\u003cspan\u003eμL Detection buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eand \u003c\/span\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eTag1-NLRP3 \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature \u003c\/span\u003e\u003cspan\u003efor 10 minutes\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e4μL \u003c\/span\u003e\u003cspan\u003eTag2-NEK7\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e*\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e10μL Antibody Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"3\" style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003eSeal the plate wells with a sealing film and incubate at room temperature \u003c\/span\u003e\u003cspan\u003efor 2\u003c\/span\u003e\u003cspan\u003e hours.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\u003cp\u003e\u003cspan\u003e*Adjust the compound addition sequence according to the inhibition mechanism of test samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3、\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eRead Data\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe detection was performed on a TR-FRET microplate reader. The excitation wavelength was 320\/340 nm, and the emission wavelengths at 620 nm and 665 nm were detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【DATA REDUCTION 】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eRatio\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e=\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(665\/620)\u003c\/span\u003e\u003cspan\u003e ×\u003c\/span\u003e\u003cspan\u003e10000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e： \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan\u003eNet signal\u003c\/span\u003e\u003cspan\u003e = (Std-\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e)\/\u003c\/span\u003e\u003cspan\u003eNC\u003c\/span\u003e\u003cspan\u003e×100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStd：the ratio of sample and control\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNC: the ratio of \u003c\/span\u003e\u003cspan\u003enegative\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econtrol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003cspan\u003e CV（%）\u003c\/span\u003e\u003cspan\u003e：\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eCV（%）= Standard Deviation\/Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e【\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003eData Example\u003c\/span\u003e\u003cspan style=\"font-weight:bold;color:#1655A5\"\u003e】\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data cannot replace experimental results obtained in experiments; \u003c\/span\u003e\u003cspan\u003ethey \u003c\/span\u003e\u003cspan\u003eare provided solely for illustrative purposes, and outcomes may vary depending on the plate reader used.\u003c\/span\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e                                         \u003c\/span\u003e\u003cspan style=\"color:#FFFFFF\"\u003e   \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/676afbee-0302-484e-af30-9da848830e23.emf\" style=\"margin-left:30px; width:315px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/5aaf0024-fb37-4c1e-a620-1de2fe1d4f8f.png\" style=\"margin-left:30px; width:404px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight:bold\"\u003eNote: Recommended microplate (384-well plate,\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ewhite, shallow wells).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":43096730992715,"sku":"UA086135-100T","price":730.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":43096731025483,"sku":"UA086135-500T","price":2180.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":43096731058251,"sku":"UA086135-2500T","price":8175.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":43096731091019,"sku":"UA086135-10000T","price":21800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_824d10a7-74c4-44d6-8586-e1598b71e1d7.png?v=1788242439","url":"https:\/\/www.antbioinc.com\/products\/unione-tr-fret-human-nek7nlrp3-binding-kit-ua086135","provider":"AntBio","version":"1.0","type":"link"}