{"product_id":"unione-tr-fret-human-cgmp-detection-kit-ua086127","title":"UniOne® TR-FRET Human cGMP Detection Kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence technology (TR-FRET)\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eenabling precise measurement of cellular cGMP production.\u003c\/span\u003e\u003cspan\u003e This method provides a high-throughput format for simple and rapid screening of molecules that modulate phosphodiesterase activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs illustrated, biotin-labeled cGMP can bind to streptavidin (SA), while the Eu-labeled anti-cGMP antibody (TR-FRET donor) and the Ac-labeled anti-Tag2 antibody (TR-FRET acceptor) bind to cGMP and biotin, respectively. The proximity of the donor and acceptor antibodies allows the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific 665 nm emission signal. When cGMP is present in cell samples or standard curves, it competes with biotin-labeled cGMP for binding to the Eu-labeled anti-cGMP antibody, thereby reducing the FRET signal. This specific signal is proportional to the degree of interaction between the Eu-labeled anti-cGMP antibody, biotin-labeled cGMP, and \u003c\/span\u003e\u003cspan\u003eSA\u003c\/span\u003e\u003cspan\u003e. This homogeneous assay is simple to perform and requires no washing steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/999c3963-fd43-4082-b051-065fb068a173.png\" style=\"margin-left:30px; width:622px; height:auto; vertical-align:middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:6.7500pt;margin-right:6.7500pt;width:510.7500pt;\" border=\"0\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" 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5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.1500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP \u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:10.0000pt;\"\u003ecalibrator\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd 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style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eAnti-cGMP Eu antibody\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:59.9000pt;\" width=\"119\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e100\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e×\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 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width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:29.6500pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:144.4000pt;\" width=\"288\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eStimulation Buffer (without IBMX)\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid 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style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:58.7500pt;\" width=\"117\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e20\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e0mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:none;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:71.4500pt;\" width=\"142\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-80℃\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003eHere is the English translation of the provided content while preserving the exact HTML structure and formatting: ```html\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e1. Reagent Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.1 Thaw all reagents to room temperature before use. The reaction system for the 384-well plate is 20μL (reagent volumes are shown in the table below). Calculate the required volume before preparation. The following preparation is for reference only, using 500 reactions as an example.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 1. Reagent Preparation and Volumes\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;width:505.0000pt;\" border=\"1\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:30.0000pt;\"\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eReagent Name\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003ePreparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"text-align:center;text-autospace:ideograph-numeric;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e\u003cstrong\u003eVolume per Well (μL)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:30.0000pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eAnti-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e Eu antibody\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"top\"\u003e\u003cp style=\"line-height:114%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eTake 25μL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eAnti-cGMP Eu antibody\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e stock solution and dilute to 2.5mL with \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well.\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5μL\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height:2.7000pt;\"\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:119.0000pt;\" width=\"238\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP Ac Mix\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:300.3500pt;\" width=\"600\" valign=\"top\"\u003e\u003cp style=\"line-height:114%;text-align:justify;text-justify:inter-ideograph;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eTake 12.5μL of Biotin\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP protein stock solution and dilute to 1.25mL with \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well; take 100μL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eSA-Ac\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e stock solution and add to 1.25mL of \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eLysis \u0026amp; Detect Buffer\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e, mix well. Mix the diluted Biotin\u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e-\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003ecGMP protein and \u003c\/span\u003e\u003c\/font\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003eSA-Ac\u003c\/span\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e in a 1:1 ratio to prepare the cGMP Ac Mix.\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:none;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:85.6500pt;\" width=\"171\" valign=\"center\"\u003e\u003cp style=\"line-height:114%;text-align:center;\"\u003e\u003cspan style=\"color:rgb(0,0,0);\"\u003e\u003cfont\u003e\u003cspan style=\"font-family:微软雅黑;font-size:9.0000pt;\"\u003e5μL\u003c\/span\u003e\u003c\/font\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.2 Gradient Dilution of Test Samples\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrepare according to experimental requirements. The following is for reference only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsing cGMP calibrator as an example, the dilution buffer is Stimulation Buffer. Adjust the sample concentration as needed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eTable 2. cGMP Calibrator Preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eFinal Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Concentration (nM)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ePreparation Method\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e1\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,250.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5,000.000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1μL of 500μM stock + 99μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e416.667\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,666.667\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ① + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e3\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e138.889\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e555.556\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ② + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e46.296\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e185.185\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ③ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e5\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e15.432\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e61.728\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ④ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e6\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5.144\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20.576\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑤ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e7\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1.715\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e6.859\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑥ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e8\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.572\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e2.286\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑦ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e9\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.191\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.762\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑧ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e10\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.064\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.254\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑨ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cspan style=\"margin-right:8px;\"\u003e11\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.021\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0.085\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e20μL of ⑩ + 40μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e0\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e40μL of 1× Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.3 Pre-Treatment for Cell-Based Experiments\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e① Stimulation Buffer is used to dilute cells and stimulant drugs. The Stimulation Buffer provided in the kit does not contain IBMX. If the experiment aims to quantify the final concentration of cGMP in cells, it is necessary to add an appropriate concentration of IBMX to prevent cGMP degradation. If the experiment aims to evaluate the performance of PDE inhibitors, IBMX should not be added.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e② Before conducting formal experiments, determine the number of cells per well and the incubation time with drugs based on cell and drug characteristics to ensure that the cGMP levels produced by cells fall within the linear range of the calibration curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Sample Addition and Controls\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 cGMP Standard Curve: 5μL of gradient-diluted cGMP reference, 5μL Stimulation Buffer, 5μL Anti-cGMP Eu antibody working solution, incubate for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.2 Test Samples: 5μL of gradient-diluted test sample, 5μL Stimulation Buffer, 5μL Anti-cGMP Eu antibody working solution, incubate for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.3 Cell-Based Experiments: Based on pre-experiment results, add 5μL of cells, 5μL of gradient-diluted drug, and incubate for the determined time. After incubation, add 5μL Anti-cGMP Eu antibody working solution, incubate at room temperature for 10min, then add 5μL cGMP Ac Mix.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.4 Negative Control (NC): 5μL Stimulation Buffer instead of test sample or 5μL of untreated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003ecGMP Standard Curve\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eTest Samples\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eCell Experiments\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"background-color:#8BAAD2;border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eNegative Control (NC)\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"4\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 1\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cGMP calibrator\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL test sample\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer or 5μL cells\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL drug※\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Stimulation Buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL Anti-cGMP Eu antibody working solution\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eIncubate at room temperature for 10min\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" rowspan=\"2\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eStep 2\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5μL cGMP Ac Mix\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\" colspan=\"4\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eSeal the plate, centrifuge at 1000rpm for 1min to mix, incubate at room temperature for 120min, then read the plate.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e•\u003c\/span\u003e\u003cspan\u003eThe incubation time of cells with drugs needs to be determined experimentally. Add subsequent reagents after drug stimulation of cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"margin-right:8px;\"\u003e3.\u003c\/span\u003e\u003cstrong\u003eDetection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMeasure using a TR-FRET-compatible microplate reader. Excitation wavelength: 320\/340nm, emission wavelengths: 620nm and 665nm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Result Calculation】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1) Calculate the signal value (Ratio): Multiply the 665nm fluorescence signal by the 620nm fluorescence signal and then by 10,000.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eRatio = (665\/620) × 10,000\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2) Calculate CV (%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eCV (%) = Standard Deviation \/ Mean Ratio × 100%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#1655A5;\"\u003e\u003cstrong\u003e【Data Example】\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following data is for reference only and may vary depending on the plate reader used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"image_resized\" style=\"height:auto;margin-left:30px;vertical-align:middle;width:492px;\" src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/6b148df9-8915-4ecf-bd83-f9ed2ecadddb.png\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color:#FFFFFF;\"\u003e3 \/3\u003c\/span\u003e\u003cstrong\u003eNote: *Data is for reference only. Recommended microplate: 384-well plate, white, shallow well.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100T","offer_id":42332771876939,"sku":null,"price":530.0,"currency_code":"USD","in_stock":true},{"title":"500T","offer_id":42332771909707,"sku":null,"price":1315.0,"currency_code":"USD","in_stock":true},{"title":"2500T","offer_id":42332771942475,"sku":null,"price":4925.0,"currency_code":"USD","in_stock":true},{"title":"10000T","offer_id":42332771975243,"sku":null,"price":13135.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_af53683f-0d09-420e-8fcc-a55757fba732.png?v=1780633793","url":"https:\/\/www.antbioinc.com\/products\/unione-tr-fret-human-cgmp-detection-kit-ua086127","provider":"AntBio","version":"1.0","type":"link"}