{"product_id":"unione-tr-fret-his-check-kit-ua086117","title":"UniOne® TR-FRET His Check kit","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e-80℃\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eThe kit employs homogeneous time-resolved fluorescence (TR-FRET) technology for the detection of His-tagged proteins. It can be used to confirm whether the His-tag motif of a protein is recognizable by anti-His antibodies or to estimate the concentration of His-tagged proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAs illustrated, the His-tagged protein is labeled with an Acceptor (Ac-Protein His Tag), while the anti-His antibody is labeled with Europium (Eu-anti-His). Upon binding of the antibody to the His-tag on the protein, the proximity between the donor and acceptor enables fluorescence resonance energy transfer (FRET), resulting in a specific 665 nm emission signal from the acceptor. In the presence of other His-tagged proteins, they compete with Ac-Protein His Tag for binding to Eu-anti-His, thereby reducing FRET signal generation. Higher concentrations of competing His-tagged proteins lead to stronger competition and lower signals. The signal intensity is proportional to the binding between Ac-Protein His Tag and Eu-anti-His, with no washing steps required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/univ-pmc.oss-cn-shanghai.aliyuncs.com\/eb946f65-2024-4944-b553-16575a47a05e.png\" style=\"margin-left: 30px; max-width: 100%; height: auto; vertical-align: middle;\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:none;margin-left:6.7500pt;margin-right:6.7500pt;width:315.2000pt;\" border=\"0\" cellspacing=\"0\"\u003e\u003ctbody\u003e\n\u003ctr style=\"height:27.8500pt;\"\u003e\n\u003ctd style=\"background-color:rgb(139,170,210);border-bottom:1.0000pt solid windowtext;border-left:1.0000pt solid windowtext;border-right:1.0000pt solid windowtext;border-top:1.0000pt solid windowtext;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;width:79.8500pt;\" 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