{"product_id":"ua-glo-ldh-luminescence-cytotoxicity-assay-ua079041","title":"UA-Glo® LDH Luminescence Cytotoxicity Assay","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLDH发光法细胞毒性检测试剂盒\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eDry ice shipment. Store away from light at -80°C, validity period as indicated on the reagent bottle label. After initial use, it is recommended to aliquot the LDH detection solution and store away from light at -20°C or below, while the reductase substrate should be aliquoted and stored away from light at -80°C to avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003eLactate dehydrogenase (LDH) is widely present in the cytoplasm of cells and is released extracellularly upon cell membrane rupture, remaining active in cell culture media for extended periods. Therefore, extracellularly released LDH is often used as an indicator of cellular health to reflect the toxic effects of external stimuli or compounds on cells. The UA-Glo® LDH Luminescent Cytotoxicity Assay Kit measures the extracellular LDH content through a coupled reaction involving LDH, reductase, and luciferase to assess cellular health.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe UA-Glo® LDH Luminescent Cytotoxicity Assay includes reductase, luciferase, and enzyme substrates, where the reductase substrate also serves as a precursor for luciferase substrate luciferin. The LDH reaction converts NAD to NADH, and the reductase utilizes NADH to convert the reductase substrate into luciferin, which is then used by luciferase to generate luminescence. The luminescence intensity is directly proportional to LDH activity, thereby indirectly determining cellular health. The UA-Glo® LDH Luminescent Cytotoxicity Assay features high signal-to-noise ratio, excellent reproducibility, and stability. Its homogeneous, ready-to-use formulation requires only a single addition, reducing potential experimental errors from multiple pipetting steps. Additionally, this assay does not require cell lysis, enabling continuous monitoring of the same cell sample and further simplifying experimental design.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe UA-Glo® LDH Luminescent Cytotoxicity Assay can be combined with other cellular health assessment reagents for multiplex detection on the same experimental sample, such as the UA-Glo® CTG Cell Viability Assay Kit (Cat# UA070103), Caspase 3\/7 Apoptosis Assay Kit (Cat# UA079012), and CTF Fluorescent Cell Viability Assay Kit (Cat# UA079015), to better elucidate the mechanisms of cytotoxicity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left;\"\u003e\u003cspan\u003eUA-Glo® LDH Luminescent Cytotoxicity Assay Kit components and specifications are as follows. The detectable number of reactions for 96-\/384-well plates is calculated based on adding 50\/10 μL of sample and 50\/10 μL of LDH detection reagent per well, respectively.\u003c\/span\u003e\u003c\/p\u003e\n\u003cfigure class=\"table\"\u003e\u003ctable style=\"border-collapse:collapse;border:1px solid #000000;width:auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e96-well plate reactions\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e384-well plate reactions\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e10ml\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eLDH detection solution: 10 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eReductase substrate: 50 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;\" lang=\"EN-US\"\u003e(1,000U\/mL)\u003c\/span\u003e\u003cspan\u003e: 200 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e200\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cstrong\u003e50ml\u003c\/strong\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eLDH detection solution: 50 mL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eReductase substrate: 250 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan style=\"font-size:10.0pt;\" lang=\"EN-US\"\u003e(1,000U\/mL)\u003c\/span\u003e\u003cspan\u003e: 200 μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e1,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #000000;text-align:center;vertical-align:middle;\"\u003e\u003cp style=\"text-align:center;\"\u003e\u003cspan\u003e5,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/figure\u003e\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e# Translation Result  \u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eUA-Glo®\u003c\/span\u003e\u003cspan\u003e  \u003c\/span\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan\u003e luminescence cytotoxicity assay kit measures extracellular\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e levels through a coupled reaction involving\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e, reductase, and luciferase to assess cell health. The luciferase reaction offers extremely high sensitivity, but parameters such as\u003c\/span\u003e\u003cspan\u003e pH\u003c\/span\u003e\u003cspan\u003e, temperature, and experimental setup significantly affect the results. Direct comparison of absolute readings is not recommended under most experimental conditions. It is advisable to include the same reference controls on each plate—for example, a cytotoxic reference compound and solvent vehicle control in tumor cell cytotoxicity assays—and normalize sample readings against these controls before data processing and comparison. The cytotoxicity (%) in the\u003c\/span\u003e\u003cspan\u003e UA-Glo®\u003c\/span\u003e\u003cspan\u003e  \u003c\/span\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan\u003e luminescence assay can be calculated using the following formula: Cytotoxicity (%) = 100 × (Sample reading – Solvent vehicle control reading) \/ (Maximum\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e release control reading – Solvent vehicle control reading). The maximum\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e release control can be either a cytotoxic reference compound or a solvent vehicle control treated with\u003c\/span\u003e\u003cspan\u003e Triton X-100\u003c\/span\u003e\u003cspan\u003e at a final concentration of\u003c\/span\u003e\u003cspan\u003e 0.2%\u003c\/span\u003e\u003cspan\u003e to lyse cells for\u003c\/span\u003e\u003cspan\u003e 10–15 min\u003c\/span\u003e\u003cspan\u003e before supernatant collection.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe kit includes a tube of\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e positive control for reagent validation and determination of the linear detection range. The\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e positive control can be diluted from the\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e stock solution (\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003esee\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e Section 1.4\u003c\/span\u003e\u003cspan\u003e) to\u003c\/span\u003e\u003cspan\u003e 30 mU\/mL\u003c\/span\u003e\u003cspan\u003e, which is the highest concentration. A\u003c\/span\u003e\u003cspan\u003e 2×\u003c\/span\u003e\u003cspan\u003e serial dilution can then be performed from\u003c\/span\u003e\u003cspan\u003e 30 mU\/mL\u003c\/span\u003e\u003cspan\u003e in\u003c\/span\u003e\u003cspan\u003e 7–8\u003c\/span\u003e\u003cspan\u003e steps to generate a standard curve.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eThe\u003c\/span\u003e\u003cspan\u003e UA-Glo®\u003c\/span\u003e\u003cspan\u003e  \u003c\/span\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan\u003e luminescence cytotoxicity assay kit is suitable for cytotoxicity testing in tumor cells, primary cells,\u003c\/span\u003e\u003cspan\u003e 3D\u003c\/span\u003e\u003cspan\u003e cell models, stem cells, and\u003c\/span\u003e\u003cspan\u003e ADCC\u003c\/span\u003e\u003cspan\u003e assays. Below is an example protocol for a compound cytotoxicity assay in a\u003c\/span\u003e\u003cspan\u003e 96-well\u003c\/span\u003e\u003cspan\u003e plate.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eSample Preparation:\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan\u003eSeed experimental cells at an appropriate density in a\u003c\/span\u003e\u003cspan\u003e 96-well\u003c\/span\u003e\u003cspan\u003e plate with\u003c\/span\u003e\u003cspan\u003e 100 µL\u003c\/span\u003e\u003cspan\u003e medium per well.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan\u003eTreat cells with compounds as required. Include a cytotoxic reference compound and solvent vehicle control on each plate for normalization.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan\u003eCollect samples for\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e measurement at multiple time points post-treatment, if needed.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan\u003eFor sampling, take\u003c\/span\u003e\u003cspan\u003e 2–5 µL\u003c\/span\u003e\u003cspan\u003e of cell culture medium and dilute\u003c\/span\u003e\u003cspan\u003e 20×\u003c\/span\u003e\u003cspan\u003e with\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eLDH\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e stock solution (200 mM Tris-HCl, pH 7.3, 10% glycerol, 1% BSA)\u003c\/span\u003e\u003cspan\u003e. Diluted samples can be tested immediately or stored at\u003c\/span\u003e\u003cspan\u003e –20°C\u003c\/span\u003e\u003cspan\u003e or below until analysis.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eDo not dilute\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e LDH\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e with medium or buffers (e.g., PBS), as this will significantly reduce\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e LDH\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e activity.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2.\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eLDH Luminescence Cytotoxicity Assay\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan\u003ePrepare the\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e detection reagent freshly before use (steps\u003c\/span\u003e\u003cspan\u003e 2)–4)\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e). For a\u003c\/span\u003e\u003cspan\u003e 96-well\u003c\/span\u003e\u003cspan\u003e plate, prepare enough reagent for\u003c\/span\u003e\u003cspan\u003e 50 µL\u003c\/span\u003e\u003cspan\u003e per sample (total\u003c\/span\u003e\u003cspan\u003e 100 µL\u003c\/span\u003e\u003cspan\u003e reaction volume). For a\u003c\/span\u003e\u003cspan\u003e 384-well\u003c\/span\u003e\u003cspan\u003e plate, prepare\u003c\/span\u003e\u003cspan\u003e 10 µL\u003c\/span\u003e\u003cspan\u003e per sample.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan\u003eThaw the\u003c\/span\u003e\u003cspan\u003e UA-Glo®\u003c\/span\u003e\u003cspan\u003e  \u003c\/span\u003e\u003cspan\u003eLDH\u003c\/span\u003e\u003cspan\u003e detection solution and reductase substrate at room temperature (\u003c\/span\u003e\u003cspan\u003e22–25°C\u003c\/span\u003e\u003cspan\u003e), then place the reductase substrate on ice.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan\u003eAfter complete thawing, equilibrate the\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e detection solution to room temperature and mix thoroughly. Luciferase reactions are sensitive to temperature fluctuations; ensure reagents and samples are equilibrated, and maintain a constant temperature (±1°C) during testing.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan\u003eThe reductase substrate is\u003c\/span\u003e\u003cspan\u003e 200×\u003c\/span\u003e\u003cspan\u003e concentrated; dilute to\u003c\/span\u003e\u003cspan\u003e 1×\u003c\/span\u003e\u003cspan\u003e in\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e detection solution to prepare the\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eLDH\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e detection reagent\u003c\/span\u003e\u003cspan\u003e. Mix thoroughly. After first use, aliquot the reductase substrate and store protected from light at\u003c\/span\u003e\u003cspan\u003e –80°C\u003c\/span\u003e\u003cspan\u003e to avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5)\u003c\/span\u003e\u003cspan\u003eRetrieve the\u003c\/span\u003e\u003cspan\u003e 20×\u003c\/span\u003e\u003cspan\u003e diluted samples. For most experiments, further dilute samples\u003c\/span\u003e\u003cspan\u003e 2×–5×\u003c\/span\u003e\u003cspan\u003e with\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e stock solution (final dilution:\u003c\/span\u003e\u003cspan\u003e 40×–100×\u003c\/span\u003e\u003cspan\u003e) before testing. Optimal dilution may vary depending on experimental parameters (e.g., cell number, serum content in medium—some serum brands contain high\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e). Pre-testing is recommended to ensure readings fall within the linear range. Refer to the \"Sample Dilution Guide\" table below for guidance.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6)\u003c\/span\u003e\u003cspan\u003eAdd\u003c\/span\u003e\u003cspan\u003e 50 µL\u003c\/span\u003e\u003cspan\u003e of diluted sample per well in a white (preferably opaque-bottom)\u003c\/span\u003e\u003cspan\u003e 96-well\u003c\/span\u003e\u003cspan\u003e plate, followed by\u003c\/span\u003e\u003cspan\u003e 50 µL\u003c\/span\u003e\u003cspan\u003e of\u003c\/span\u003e\u003cspan\u003e LDH\u003c\/span\u003e\u003cspan\u003e detection reagent.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7)\u003c\/span\u003e\u003cspan\u003eMix by shaking at medium speed for\u003c\/span\u003e\u003cspan\u003e 1 min\u003c\/span\u003e\u003cspan\u003e, then incubate in the dark for\u003c\/span\u003e\u003cspan\u003e 30–60 min\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8)\u003c\/span\u003e\u003cspan\u003eMeasure luminescence using a plate reader. Readings can be taken after\u003c\/span\u003e\u003cspan\u003e 60 min\u003c\/span\u003e\u003cspan\u003e, but ensure signals remain within the linear range and are still increasing.\u003c\/span\u003e\u003c\/p\u003e  \u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSample Dilution Guide\u003c\/span\u003e\u003c\/p\u003e  \u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e  \u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eMedium Composition\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eCell Number\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e(100 µL)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e1) Different batches are not recommended for mixed use  2) Without strict validation, it is not recommended to alter the dosage of detection reagents  3) For research use only\u003c\/p\u003e\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10ml","offer_id":43093313355851,"sku":"UA079041-10ml","price":316.67,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":43093313388619,"sku":"UA079041-50ml","price":1146.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/6e470891-56cc-4af4-91c8-d582a1d97523.png?v=1788188482","url":"https:\/\/www.antbioinc.com\/products\/ua-glo-ldh-luminescence-cytotoxicity-assay-ua079041","provider":"AntBio","version":"1.0","type":"link"}