{"product_id":"ua-glo-cell-apoptosis-live-multiplex-assay-ua079064","title":"UA-Glo® Cell Apoptosis-Live Multiplex Assay","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003e\u003cspan\u003eDry ice transport. Store protected from light at −20°C or lower; for long-term storage (\u0026gt;2 months), we recommend −80°C. Refer to the reagent vial label for the expiration date. After first use, it is recommended to aliquot the reagent and store it protected from light at −20°C or lower to avoid repeated freeze-thaw cycles.\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003e\u003cspan\u003eThe UA-Glo® Cell Viability and Apoptosis Multi-Assay Kit can assess both cell viability and the apoptotic status of the same cell sample, thereby enabling a more accurate and efficient determination of the mechanisms underlying cytotoxicity. The kit's viability assay is based on the cleavage of a membrane-permeable peptide-fluorophore substrate by an enzyme that is active only in live cells, resulting in the release of the fluorophore and the generation of a fluorescent signal; the fluorescence intensity is proportional to the number of viable cells. When cytotoxicity compromises cell membrane integrity, this enzyme is released into the extracellular space and loses its activity, leading to no fluorescence signal. The apoptosis assay detects Caspase-3\/7 activity within cells as an indicator of apoptosis. After completing the viability assay, the Caspase-3\/7 detection reagent can be directly added to the cell sample; the intracellular Caspases 3\/7 cleave the Caspase-3\/7–specific peptide-luciferin substrate in the reagent, releasing luciferin. The luciferase in the reagent then catalyzes a luciferin reaction, producing light whose intensity is proportional to the activity of Caspases 3\/7. By employing two compatible biochemical detection principles, the UA-Glo® Cell Viability and Apoptosis Multi-Assay Kit allows for sequential assessment of cell viability and apoptosis in the same sample, simplifying experimental design, saving both samples and experimental time. Its straightforward \"add-sample-mix-detect\" workflow and stable detection signals make it particularly suitable for high-throughput screening of compounds.\u003c\/span\u003e\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003eUA-Glo®\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eThe components and specifications of the cell viability and apoptosis multiplex assay kit are as follows. Among them,\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003eOrifice plate and\u003c\/span\u003e\u003cspan\u003e384-\u003c\/span\u003e\u003cspan\u003eThe number of detection reactions per well is calculated based on the culture medium volume of each well as follows:\u003c\/span\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003e20 μL\u003c\/span\u003e\u003cspan\u003eAdd the cell viability assay reagent to each well, respectively.\u003c\/span\u003e\u003cspan\u003eCaspase-3\/7\u003c\/span\u003e\u003cspan\u003eTesting reagent\u003c\/span\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eμL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\/100 μL\u003c\/span\u003e\u003cspan\u003eand\u003c\/span\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\/25 μL\u003c\/span\u003e\u003cspan\u003eCalculation.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse:collapse;width:auto;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eSpecifications\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eComponents and specifications\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e96-\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNumber of orifices in the orifice plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003eDetection\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e384-\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003eNumber of orifices in the orifice plate\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e10 ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e5 mL\u003c\/span\u003e\u003cspan\u003eCell viability assay solution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003eCell viability assay substrate\u003c\/span\u003e\u003cspan style=\"color:#EE0000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e10 mL Caspase-3\/7\u003c\/span\u003e\u003cspan\u003eTesting reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e400\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan style=\"font-weight:bold\"\u003e5 x 10 ml\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e15 mL\u003c\/span\u003e\u003cspan\u003eCell viability assay solution\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003eCell viability assay substrate\u003c\/span\u003e\u003cspan style=\"color:#EE0000\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan\u003e5 x 10 mL Caspase-3\/7\u003c\/span\u003e\u003cspan\u003eTesting reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e500\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;vertical-align:middle;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000;border:1px solid #000000\"\u003e\u003cp style=\"text-align:center\"\u003e\u003cspan\u003e2,000\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold\"\u003eReagent preparation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan\u003eAfter the cell viability assay solution and the cell viability substrate have completely melted, equilibrate to\u003c\/span\u003e\u003cspan\u003e37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003eVortex the cell viability assay substrate to ensure thorough mixing, then briefly centrifuge to collect the substrate at the bottom of the tube.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e2)\u003c\/span\u003e\u003cspan\u003ePrepare the cell viability assay reagent.\u003c\/span\u003e\u003cspan\u003e(5x)\u003c\/span\u003e\u003cspan\u003e: The substrate concentration for cell viability assay is\u003c\/span\u003e\u003cspan\u003e100 mM\u003c\/span\u003e\u003cspan\u003e, soluble in\u003c\/span\u003e\u003cspan\u003eDMSO\u003c\/span\u003e\u003cspan\u003eIn medium. Prepare the cell viability assay reagent by mixing the cell viability assay solution and the substrate according to the following ratio, based on the required assay volume.\u003c\/span\u003e\u003cspan\u003e(5x)\u003c\/span\u003e\u003cspan\u003e: For\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003eOrifice plate test board inspection,\u003c\/span\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003eThe substrate is added to\u003c\/span\u003e\u003cspan\u003e2 mL\u003c\/span\u003e\u003cspan\u003eIn the cell viability assay solution; for\u003c\/span\u003e\u003cspan\u003e384-\u003c\/span\u003e\u003cspan\u003eKong test board inspection,\u003c\/span\u003e\u003cspan\u003e10 μL\u003c\/span\u003e\u003cspan\u003eThe substrate is added to\u003c\/span\u003e\u003cspan\u003e2.5 mL\u003c\/span\u003e\u003cspan\u003eIn the cell viability assay solution, shake vigorously to ensure thorough mixing. After preparation, the cell viability assay reagent can be stored at room temperature in the dark.\u003c\/span\u003e\u003cspan\u003e24hr\u003c\/span\u003e\u003cspan\u003eUse within. Store the remaining cell viability assay reagent protected from light.\u003c\/span\u003e\u003cspan\u003e4\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUse within 7 days.\u003c\/span\u003e\u003cspan\u003e3)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003eCaspase-3\/7\u003c\/span\u003e\u003cspan\u003eAfter the assay reagent has completely melted, equilibrate to room temperature and mix by inverting. Take the required amount for direct use. Unused\u003c\/span\u003e\u003cspan\u003eCaspase-3\/7\u003c\/span\u003e\u003cspan\u003eassay reagent should be aliquoted as needed and stored at\u003c\/span\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003eand below, avoiding repeated freeze-thaw cycles. For long-term storage\u003c\/span\u003e\u003cspan\u003e(\u0026gt;2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003emonths\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003eit is recommended to store at\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e-80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003efor\u003c\/span\u003e\u003cspan\u003elonger periods.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"font-weight:bold\"\u003eThe following examples are based on experiments using a\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003e96-\u003c\/span\u003e\u003cspan style=\"font-weight:bold\"\u003ewell\/384-well (numbers in parentheses) cell culture plate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e1)\u003c\/span\u003e\u003cspan\u003eSeed cells at an appropriate density into a 96-well cell culture plate; it is recommended to use a white-framed transparent cell culture plate.\u003c\/span\u003e\u003cspan\u003e2)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003eAdd compounds at an appropriate concentration or gradient concentration to the cells. The final volume of the medium after adding the compound should be\u003c\/span\u003e\u003cspan\u003e100 μL (20 μL),\u003c\/span\u003e\u003cspan\u003ewith the organic solvent concentration kept below\u003c\/span\u003e\u003cspan\u003e1%.\u003c\/span\u003e\u003cspan\u003e1%\u003c\/span\u003e\u003cspan\u003eContinue incubation for an appropriate time according to experimental requirements.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"font-weight:bold\"\u003eExperimental control\u003c\/span\u003e\u003cspan\u003eIt is recommended to include at least a cell control treated with the solvent carrier alone, as well as a culture medium control treated with the solvent carrier alone.\u003c\/span\u003e\u003cspan\u003e(\u003c\/span\u003e\u003cspan\u003eBlank control\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003eand positive compound controls. The blank control is the cell culture system's response to the cell viability assay reagent and\u003c\/span\u003e\u003cspan\u003eCaspase 3\/7\u003c\/span\u003e\u003cspan\u003eFor background correction of the assay reagents, you may subtract the blank control value from the measured values before performing subsequent calculations. The positive compound control reflects the maximal effect of the compound. The cell control reflects the viability of cells treated with the solvent vehicle and the baseline cellular activity.\u003c\/span\u003e\u003cspan\u003eCaspase 3\/7\u003c\/span\u003e\u003cspan\u003eActivity; the two can be used to normalize the experimental results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e3)\u003c\/span\u003e\u003cspan\u003eRemove the experimental cell culture plate,\u003c\/span\u003e\u003cspan\u003e96-\u003c\/span\u003e\u003cspan\u003ePer well of the porous cell culture plate\u003c\/span\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(20 μL)\u003c\/span\u003e\u003cspan\u003eAdd the culture medium.\u003c\/span\u003e\u003cspan\u003e20 μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(5 μL) 5x\u003c\/span\u003e\u003cspan\u003eCell viability assay reagent\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e4)\u003c\/span\u003e\u003cspan\u003eLow-speed vibration plate\u003c\/span\u003e\u003cspan\u003e20s\u003c\/span\u003e\u003cspan\u003eMix well, protect from light.\u003c\/span\u003e\u003cspan\u003e37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eReaction\u003c\/span\u003e\u003cspan\u003e30 minutes\u003c\/span\u003e\u003cspan\u003eSubsequently, fluorescence detection is performed. Different cell types exhibit varying rates of substrate cleavage in cell viability assays; therefore, the optimal detection time may need to be optimized based on the specific cell type and experimental conditions.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe fastest it can be is when the reagent is added.\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e30 minutes\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThen proceed with reading the board. Generally, at\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e60\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003eThe signal-to-noise ratio of the results is higher after a measurement period of several minutes or longer. It is recommended that the plate reading time not exceed\u003c\/span\u003e\u003cspan style=\"color:#000000\"\u003e3hr\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e5)\u003c\/span\u003e\u003cspan\u003eOn the microplate reader, it is displayed as\u003c\/span\u003e\u003cspan\u003eEx 400 nm\/Em 505 nm\u003c\/span\u003e\u003cspan\u003eDetect fluorescent signal\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e6)\u003c\/span\u003e\u003cspan\u003eAfter completing the cell viability assay, equilibrate the experimental cell culture plate to room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:justify\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e7)\u003c\/span\u003e\u003cspan\u003eAdd to each well of the experimental cell culture plate:\u003c\/span\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e(25 μL) Caspase-3\/7\u003c\/span\u003e\u003cspan\u003eTesting reagents, low-speed plate shaker\u003c\/span\u003e\u003cspan\u003e20s\u003c\/span\u003e\u003cspan\u003eIncubate in the dark at room temperature.\u003c\/span\u003e\u003cspan\u003e30-60 minutes\u003c\/span\u003e\u003cspan\u003e. The detection signal is generally applied after\u003c\/span\u003e\u003cspan\u003eCaspase-3\/7\u003c\/span\u003e\u003cspan\u003eTesting reagent\u003c\/span\u003e\u003cspan\u003e30 min - 60 min\u003c\/span\u003e\u003cspan\u003eReaches its maximum value. It can occur as early as after the addition of the detection reagent.\u003c\/span\u003e\u003cspan\u003e20-30 minutes\u003c\/span\u003e\u003cspan\u003eRead the plate; do not exceed\u003c\/span\u003e\u003cspan\u003e3 hours\u003c\/span\u003e\u003cspan\u003eat the latest. The optimal detection time depends on the cells and experimental setup and may need to be optimized based on the specific experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align:left\"\u003e\u003cspan style=\"margin-right: 8px;\"\u003e8)\u003c\/span\u003e\u003cspan\u003eDetect the luminescence signal using a microplate reader.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003eDo not mix different batches.\u003cbr\u003eDo not alter the volume of the test reagent without rigorous validation.\u003cbr\u003eFor research use only.\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"10ml","offer_id":67469884325963,"sku":"UA079064-10ml","price":410.0,"currency_code":"USD","in_stock":true},{"title":"5*10ml","offer_id":67469884358731,"sku":"UA079064-5*10ml","price":1300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/AntBioImage_7e657a6d-2e4c-4fe5-a30c-9fe7c9cc2234.png?v=1794596415","url":"https:\/\/www.antbioinc.com\/products\/ua-glo-cell-apoptosis-live-multiplex-assay-ua079064","provider":"AntBio","version":"1.0","type":"link"}