{"product_id":"tsa-buffer-abs90018","title":"TSA Buffer","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. After slicing or chip samples until the step of incubating polyHRP secondary antibody is completed, wash PBS three times for 5min each time;\u003cbr\u003e2. The concentrated fluorescent dye and the TSA buffer are uniformly mixed according to the ratio of 1:50-1: 400 to prepare the TSA fluorescent dye reaction solution;\u003cbr\u003e3. Add the prepared TSA fluorescent dye reaction solution dropwise to the sections to evenly cover the tissue at room temperature for 1-15min (the best time is 5min-10min), and wash it three times with PBS (in the pre-experiment, you can first dye for 1min to wash the fluorescent dye and then observe the staining effect under a microscope. If the positive is weak, continue to add fluorescent dye dropwise to strengthen the staining intensity until the appropriate intensity, and then continue to the next step).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThis product is one of the components of multiplex immunofluorescence kit, which is used for dilution of concentrated dyes. TSA fluorescent dye reaction solution = TSA xxx fluorescent dye + TSA buffer; Wherein TSA xxx fluorescent dye: TSA buffer = 1: 100; The dilution ratio of TSA xxx fluorescent dye and TSA buffer can be flexibly adjusted and optimized according to specific conditions, and the optimal range is 1:50-1: 400 (1: 100 can get the best results in most cases); It is generally recommended that if the primary antibody incubation time is within 1h-3h at room temperature, the recommended dilution ratio is 1:50-1: 200, and if the primary antibody incubation time is 4 overnight, the recommended dilution ratio is 1: 200-1: 400 or higher.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGeneral Notes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1. Slice or chip samples until the step of incubating polyHRP secondary antibody is completed, and wash PBS three times for 5 minutes each time.\u003cbr\u003e2. This product is only for scientific research and must not be used for clinical diagnosis or treatment.\u003cbr\u003e3. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temp.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eStored at 4 ℃, shelf life is 1 year.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003c\/div\u003e","brand":"Absin","offers":[{"title":"10mL","offer_id":43214308704331,"sku":"abs90018-10mL","price":50.0,"currency_code":"USD","in_stock":true},{"title":"50mL","offer_id":43214308737099,"sku":"abs90018-50mL","price":200.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.antbioinc.com\/products\/tsa-buffer-abs90018","provider":"AntBio","version":"1.0","type":"link"}