{"product_id":"trypsin-mass-spectrometry-grade-ua070085","title":"Trypsin, Mass Spectrometry Grade","description":"\u003ch4\u003eProduct Specification\u003c\/h4\u003e\u003cdiv class=\"responsive-table product-detail-table details-table\"\u003e\n\u003cbr\u003e\u003ctable style=\"width: 100%; height: auto;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eSynonyms\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eTrypsin(pig); porcine pancreas; Proenzyme; Parenzymol; PseudotrypsinTryptar; Trypure; Trypsin，Mass Spectrometry Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eExpression System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003ePichia pastoris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e24±2 kDa (Reducing)\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e＞98% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eNo Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003ePhysical Appearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003e2mM HCl，2mM Ca2+，ph3.0  @ 25°C\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp style=\"margin-bottom: 0px;\"\u003eTrypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of 2mM HCl. \u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eStability \u0026amp; Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eStore at -25 ~ -15℃ for 2 years\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%;\"\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 78%;\"\u003e\u003cp\u003eOlsen, J. V . Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues[J]. Molecular \u0026amp; Cellular Proteomics, 2004, 3(6):608-614.\u003cbr\u003eChi W J. Medium Optimization and Application of Affinity Column Chromatography for Trypsin Production from Recombinant Streptomyces griseus[J].Journal of Microbiology \u0026amp; Biotechnology, 2009, 19(10):1191-1196.\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003c\/div\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eTrypsin is a serine protease commonly found in the vertebrate digestive system that hydrolyzes proteins and is secreted in the pancreas as inactive trypsinogen. Its site of cleavage of the peptide chain is located primarily at the carboxyl terminus of lysine or arginine (except when both are immediately followed by proline). Trypsin consists of two subunits, the alpha subunit (with 2 polypeptide chains forming it) and the beta subunit (with 1 polypeptide chain forming it). Its hydrolytic activity can be inhibited by a variety of inhibitors, including 1) organophosphorus compounds, such as isopropyl fluorophosphate; 2) natural trypsin inhibitors derived from pancreas, soybeans, green beans, and egg whites; 3) silver ions; and 4) specific protease inhibitors, such as AEBSF, anti-protease, peptidylase, DFP, leucoinhibitory peptidase, PMSF, TLCK, etc.\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eComponents\u003c\/h4\u003e\u003cdiv\u003e\u003cp\u003eTrypsin，Mass Spectrometry Grade lyophilized\u003cbr\u003e2*Reaction buffer:，100 mM Tris-HCl 、40 mM CaCl2  pH 8 @ 25°C\u003c\/p\u003e\u003c\/div\u003e\u003ch4\u003eProtocol\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp\u003e1 Trypsin, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of 2mM HCl\u003cbr\u003e2 Set-up a typical reaction as follows\u003cbr\u003e1）Add the following components in sequence\u003c\/p\u003e\n\u003ctable class=\"table table-bordered\" style=\"width: 592.34px;\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 312.6px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 279.007px;\"\u003e\u003cp\u003e\u003cb\u003e\u003cspan style=\"font-weight:bold;font-size:10.5000pt\"\u003eVolume\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eSubstrate protein\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e5 μl (about 2 ug)\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003eddH2O\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e4.5μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e2*Reaction buffer\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e10μl\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e100 ng\/μl Tyrpsin \u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 596.4px;\"\u003e\u003cp\u003e\u003cspan style=\"font-size:10.5000pt\"\u003e0.5 μL\u003c\/span\u003e\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e2）Incubate at 37°C for 2~4h\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eGuidelines\u003c\/h4\u003e\u003cdiv\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e1. Substrate must be in phosphate-free buffer to prevent calcium precipitation with both reconstituted enzyme and enzyme buffer.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0px;\"\u003e2. Storage buffer:2mM HCl, pH3.0.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch4\u003eUnit Definition\u003c\/h4\u003e\u003cdiv\u003eIn a 3.2ml reaction system using BAEE as substrate, the UV absorption at 253nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit\u003c\/div\u003e","brand":"UA BIOSCIENCE","offers":[{"title":"100µg","offer_id":43030052798539,"sku":"UA070085-100µg","price":85.0,"currency_code":"USD","in_stock":true},{"title":"200µg","offer_id":43030052831307,"sku":"UA070085-200µg","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/8375\/1499\/files\/207c3b63bae94e878ac9d283f3f37c41.png?v=1786474829","url":"https:\/\/www.antbioinc.com\/products\/trypsin-mass-spectrometry-grade-ua070085","provider":"AntBio","version":"1.0","type":"link"}